Abstract
Rats raised in an enriched condition (EC) during development display increased hyperactivity to the effect of acute amphetamine compared to rats raised in an impoverished condition (IC). The present study determined whether environmental enrichment differentially alters the effects of GBR 12935 administration, a selective dopamine transporter (DAT) inhibitor. Acutely, EC rats showed a greater, dose-dependent GBR 12935-induced increase in activity compared to IC rats; however, basal activity for EC rats was lower than for IC rats. After repeated GBR 12935, only EC rats exhibited behavioral sensitization. Kinetic analysis of DAT function in medial prefrontal cortex (mPFC) revealed that the maximal velocity of [3H]dopamine ([3H]DA) uptake in EC rats was less than in IC rats (4.9±0.6 and 7.7±0.6pmol/min/ mg, respectively), but not in striatum or nucleus accumbens. Furthermore, GBR 12935-induced inhibition of DAT function, [3H]GBR 12935 binding density and DA content in mPFC, striatum and nucleus accumbens were not different between EC and IC rats. However, dihydroxyphenylacetic acid content in mPFC was lower in EC than IC rats, whereas no differences were found in striatum and nucleus accumbens. These results suggest that EC-induced changes in activity may be due to decreased DAT function and decreased DA metabolism in the mPFC.
Original language | English |
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Pages (from-to) | 107-117 |
Number of pages | 11 |
Journal | Behavioural Brain Research |
Volume | 148 |
Issue number | 1-2 |
DOIs | |
State | Published - Jan 5 2004 |
Bibliographical note
Funding Information:This research was supported by NIH Grants DA12964, DA05312, DA06093 and DA00399.
Keywords
- Activity
- Dopamine transporter
- Dopamine uptake
- Environmental enrichment
- GBR 12935
- Medial prefrontal cortex
ASJC Scopus subject areas
- Behavioral Neuroscience