Enzymatic strategies and biocatalysts for amide bond formation: Tricks of the trade outside of the ribosome

Anwesha Goswami, Steven G. Van Lanen

Research output: Contribution to journalReview articlepeer-review

86 Scopus citations


Amide bond-containing (ABC) biomolecules are some of the most intriguing and functionally significant natural products with unmatched utility in medicine, agriculture and biotechnology. The enzymatic formation of an amide bond is therefore a particularly interesting platform for engineering the synthesis of structurally diverse natural and unnatural ABC molecules for applications in drug discovery and molecular design. As such, efforts to unravel the mechanisms involved in carboxylate activation and substrate selection has led to the characterization of a number of structurally and functionally distinct protein families involved in amide bond synthesis. Unlike ribosomal synthesis and thio-templated synthesis using nonribosomal peptide synthetases, which couple the hydrolysis of phosphoanhydride bond(s) of ATP and proceed via an acyl-adenylate intermediate, here we discuss two mechanistically alternative strategies: ATP-dependent enzymes that generate acylphosphate intermediates and ATP-independent transacylation strategies. Several examples highlighting the function and synthetic utility of these amide bond-forming strategies are provided.

Original languageEnglish
Pages (from-to)338-353
Number of pages16
JournalMolecular BioSystems
Issue number2
StatePublished - Feb 1 2015

Bibliographical note

Publisher Copyright:
© The Royal Society of Chemistry 2015.

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology


Dive into the research topics of 'Enzymatic strategies and biocatalysts for amide bond formation: Tricks of the trade outside of the ribosome'. Together they form a unique fingerprint.

Cite this