TY - JOUR
T1 - Enzymatic total synthesis of defucogilvocarcin M and its implications for gilvocarcin biosynthesis
AU - Pahari, Pallab
AU - Kharel, Madan K.
AU - Shepherd, Micah D.
AU - Van Lanen, Steven G.
AU - Rohr, Jürgen
PY - 2012/1/27
Y1 - 2012/1/27
N2 - Teamwork: Defucogilvocarcin M (1, see scheme) was synthesized in a one-pot, enzymatic reaction from acetyl-CoA and malonyl-CoA by a combination of 15 enzymes obtained from E. coli as well as the gilvocarcin, jadomycin, and ravidomycin biosynthetic pathways. The mixture of enzymes was systematically reduced and varied to further delineate the sequence of reactions in the complex, post-polyketide steps of gilvocarcin biosynthesis.
AB - Teamwork: Defucogilvocarcin M (1, see scheme) was synthesized in a one-pot, enzymatic reaction from acetyl-CoA and malonyl-CoA by a combination of 15 enzymes obtained from E. coli as well as the gilvocarcin, jadomycin, and ravidomycin biosynthetic pathways. The mixture of enzymes was systematically reduced and varied to further delineate the sequence of reactions in the complex, post-polyketide steps of gilvocarcin biosynthesis.
KW - biosynthesis
KW - defucogilvocarcin M
KW - enzymes
KW - gilvocarcins
KW - total synthesis
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U2 - 10.1002/anie.201105882
DO - 10.1002/anie.201105882
M3 - Article
C2 - 22223167
AN - SCOPUS:84856179567
SN - 1433-7851
VL - 51
SP - 1216
EP - 1220
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 5
ER -