Enzymatic total synthesis of defucogilvocarcin M and its implications for gilvocarcin biosynthesis

Pallab Pahari, Madan K. Kharel, Micah D. Shepherd, Steven G. Van Lanen, Jürgen Rohr

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Teamwork: Defucogilvocarcin M (1, see scheme) was synthesized in a one-pot, enzymatic reaction from acetyl-CoA and malonyl-CoA by a combination of 15 enzymes obtained from E. coli as well as the gilvocarcin, jadomycin, and ravidomycin biosynthetic pathways. The mixture of enzymes was systematically reduced and varied to further delineate the sequence of reactions in the complex, post-polyketide steps of gilvocarcin biosynthesis.

Original languageEnglish
Pages (from-to)1216-1220
Number of pages5
JournalAngewandte Chemie - International Edition
Volume51
Issue number5
DOIs
StatePublished - Jan 27 2012

Keywords

  • biosynthesis
  • defucogilvocarcin M
  • enzymes
  • gilvocarcins
  • total synthesis

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry

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