Abstract
Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass — methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.
Original language | English |
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Pages (from-to) | 290-298 |
Number of pages | 9 |
Journal | Current Opinion in Biotechnology |
Volume | 69 |
DOIs | |
State | Published - Jun 2021 |
Bibliographical note
Funding Information:We gratefully acknowledge the support of R37 AI52218, R01 GM115261, the Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation (CPRI, N.I.H.P20 GM130456), the University of Kentucky College of Pharmacy and the National Center for Advancing Translational Sciences (UL1TR000117 and UL1TR001998).
Funding Information:
We gratefully acknowledge the support of R37 AI52218 , R01 GM115261 , the Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation ( CPRI , N.I.H. P20 GM130456 ), the U niversity of Kentucky College of Pharmacy and the National Center for Advancing Translational Sciences ( UL1TR000117 and UL1TR001998 ).
Publisher Copyright:
© 2021 Elsevier Ltd
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering