TY - JOUR
T1 - Enzyme-mediated bioorthogonal technologies
T2 - catalysts, chemoselective reactions and recent methyltransferase applications
AU - Jalali, Elnaz
AU - Thorson, Jon S.
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/6
Y1 - 2021/6
N2 - Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass — methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.
AB - Transferases have emerged as among the best catalysts for enzyme-mediated bioorthogonal functional group installation to advance innovative in vitro, cell-based and in vivo chemical biology applications. This review introduces the key considerations for selecting enzyme catalysts and chemoselective reactions most amenable to bioorthogonal platform development and highlights relevant key technology development and applications for one ubiquitous transferase subclass — methyltransferases (MTs). Within this context, recent advances in MT-enabled bioorthogonal labeling/conjugation relevant to DNA, RNA, protein, and natural products (i.e. complex small molecule metabolites) are highlighted.
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U2 - 10.1016/j.copbio.2021.02.010
DO - 10.1016/j.copbio.2021.02.010
M3 - Review article
C2 - 33901763
AN - SCOPUS:85104681842
SN - 0958-1669
VL - 69
SP - 290
EP - 298
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -