Epididymal Fat-Derived Sympathoexcitatory Signals Exacerbate Neurogenic Hypertension in Obese Male Mice Exposed to Early Life Stress

Carolina Dalmasso, Jacqueline R. Leachman, Sundus Ghuneim, Nermin Ahmed, Eve R. Schneider, Olivier Thibault, Jeffrey L. Osborn, Analia S. Loria

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Previously, we have shown that male mice exposed to maternal separation and early weaning (MSEW) - a mouse model of early life stress - display increased mean arterial pressure compared with controls when fed a high-fat diet. As the stimulation of sensory nerves from fat has been shown to trigger the adipose afferent reflex, we tested whether MSEW male mice show obesity-associated hypertension via the hyperactivation of this sympathoexcitatory mechanism. After 16 weeks on high-fat diet, MSEW mice displayed increased blood pressure, sympathetic activation, and greater depressor response to an α-adrenergic blocker when compared with controls (P<0.05; n=8), despite no differences in adiposity and plasma leptin. The acute infusion of capsaicin in epididymal white adipose tissue (1.5 pmol/μL of capsaicin, 8 μL/per site, 4 sites, bilaterally) increased the total pressor response in MSEW mice compared with controls (110±19 versus 284±33 mm Hg×30 minutes; P<0.05; n=8). This response was associated with neuronal activation in OVLT, posterior paraventricular nucleus of the hypothalamus, and RVLM (P<0.05 versus control; n=6-7). Renal denervation abolished both the acute and chronic elevated mean arterial pressure in obese MSEW mice. Moreover, selective sensory denervation of epididymal white adipose tissue using resiniferatoxin (10 pmol/µL solution; n=6) decreased mean arterial pressure in obese MSEW mice only (P<0.05 versus control). Obese MSEW mice displayed increased epididymal white adipose tissue levels of both tryptophan hydroxylase (Tph1) mRNA expression and its synthesis product serotonin (8.3±1.9 versus 16.6±1.7 ug/mg tissue; P<0.05 versus control). Thus, afferent sensory signals from epididymal white adipose tissue may contribute to the exacerbated fat-brain-blood pressure axis displayed by obese male mice exposed to early life stress.

Original languageEnglish
Pages (from-to)1434-1449
Number of pages16
Issue number5
StatePublished - Nov 1 2021

Bibliographical note

Publisher Copyright:
© 2021 American Heart Association, Inc.


  • adiposity
  • blood pressure
  • capsaicin
  • leptin
  • serotonin

ASJC Scopus subject areas

  • Internal Medicine


Dive into the research topics of 'Epididymal Fat-Derived Sympathoexcitatory Signals Exacerbate Neurogenic Hypertension in Obese Male Mice Exposed to Early Life Stress'. Together they form a unique fingerprint.

Cite this