Epigenetic effects induced by silver nanoparticles in Caenorhabditis elegans after multigenerational exposure

Anye Wamucho, Allison Heffley, Olga V. Tsyusko

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Multigenerational effects of silver nanoparticles (Ag-NPs) on reproduction of the soil nematode Caenorhabditis elegans have been observed previously. However, mechanisms of this reproductive sensitivity are unknown. Here we examine whether epigenetic changes occur as a result of multigenerational exposure to Ag-NPs and whether such modifications can be inherited by unexposed generations. Changes at histone methylation markers, histone 3 lysine 4 dimethylation (H3K4me2) and histone 3 lysine 9 trimethylation (H3K9me3), known to affect reproduction, as well as changes in the expression of the genes encoding demethylases and methyltransferases associated with the selected markers, were investigated. We exposed C. elegans at EC30 to AgNO3, pristine Ag-NPs, and its environmentally transformed product, sulfidized Ag-NPs (sAg-NPs). Histone methylation levels at H3K4me2 increase in response to pristine Ag-NP exposure and did not recover after rescue from the exposure, suggesting transgenerational inheritance. Compared to pristine Ag-NPs, exposure to transformed sAg-NPs significantly decreased H3K4me2 and H3K9me3 levels. These changes in the histone methylation were also supported by expression of spr-5 and jmjd-2 (H3K4me2 and H3K9me3 demethylases, respectively) and set-30 (H3K4me2 methyltransferase). Our study demonstrates that multigenerational exposure to Ag-NPs induces epigenetic changes that are inherited by unexposed offspring. However, environmental transformations of Ag-NPs may also reduce toxicity via epigenetic mechanisms, such as changes at histone methylation.

Original languageEnglish
Article number138523
JournalScience of the Total Environment
Volume725
DOIs
StatePublished - Jul 10 2020

Bibliographical note

Funding Information:
The authors gratefully acknowledge assistance of Jason Unrine, Shristi Shrestha, Stuart Lichtenberg, Tyler Bair, Jieran Li, and Zeinah El Baddar. Caenorhabditis elegans strains were provided by the Caenorhabditis Genetics Center, which is funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). This work was supported by the U.S. National Science Foundation (NSF) and U.S. Environmental Protection Agency (EPA) under EF-0830093 , DBI-1266252 , and CBET- 1530594 . This work was also supported by the USDA National Institute of Food and Agriculture Multistate Project NC 1194. Any opinions, findings, conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the NSF or the EPA. This work has not been subjected to EPA review and no official endorsement should be inferred. AH was supported by Summer Undergraduate Research in Environmental Sciences (SURES) funded by the National Institute of Environmental Health Sciences (NIEHS) R25ES027684 .

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • Demethylase
  • Gene expression
  • Histone methylation
  • Methyltransferase
  • Nanomaterials
  • Nematode

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

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