Background: Epigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiological processes. However, our current understanding of epigenetic regulation of JH action is still limited. Hence, we studied the role of CREB binding protein (CBP, contains HAT domain) and Trichostatin A (TSA, HDAC inhibitor) on JH action. Results: Exposure of Tribolium castaneum cells (TcA cells) to JH or TSA caused an increase in expression of Kr-h1 (a known JH-response gene) and 31 or 698 other genes respectively. Knockdown of the gene coding for CBP caused a decrease in the expression of 456 genes including Kr-h1. Interestingly, the expression of several genes coding for transcription factors, nuclear receptors, P450 and fatty acid synthase family members that are known to mediate JH action were affected by CBP knockdown or TSA treatment. Conclusions: These data suggest that acetylation and deacetylation mediated by HATs and HDACs play an important role in JH action.
|State||Published - Dec 14 2018|
Bibliographical noteFunding Information:
This work is supported by a grant from the National Institutes of Health (GM070559–11) and the National Institute of Food and Agriculture, USDA, HATCH under 2351177000. AR is supported by EXTEMIT - K, No. CZ. 02. 1.01/ 0.0/0.0/15_003/0000433 financed by OP RDE during the preparation of part of the manuscript.
© 2018 The Author(s).
- FOXO Tribolium and TcA cells
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