TY - JOUR
T1 - Epithelial cell-enhanced metabolism by low-level laser therapy and epidermal growth factor
AU - Basso, Fernanda Gonçalves
AU - Pansani, Taisa Nogueira
AU - Cardoso, Lais Medeiros
AU - Citta, Mariana
AU - Soares, Diana Gabriela
AU - Scheffel, Débora Salles
AU - Hebling, Josimeri
AU - de Souza Costa, Carlos Alberto
N1 - Publisher Copyright:
© 2017, Springer-Verlag London.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Reepithelialization and wound closure are the desired outcome for several ulcerative conditions. Such resolution reduces the possibility of wound contamination and maintenance of the injury and improves the reestablishment of tissue morphology and functions. Investigators are seeking adjuvant therapies that can accelerate wound healing and are developing new strategies for clinical applications. This study compared the effects of epidermal growth factor (EGF) application and low-level laser therapy (LLLT) on cultured epithelial cells. Cells were seeded in 24-well plates. After a 24-h incubation, the epithelial cells were either treated with EGF (100 μM in serum-free DMEM for 72 h) or subjected to LLLT (780 nm, 25 mW, 0.5, 1.5, and 3 J/cm2) by three applications every 24 h. Seventy-two hours after cells were treated with EGF or LLLT, cell migration, viability, proliferation, and collagen synthesis were assessed. Cells treated with EGF showed increased cell viability, proliferation, and collagen synthesis compared with those cells that received no treatment. LLLT enhanced cell migration; however, no significant effects of laser irradiation on other cell functions were observed. Comparison of both therapies demonstrated that EGF and LLLT enhanced specific epithelial cell activities related to wound healing.
AB - Reepithelialization and wound closure are the desired outcome for several ulcerative conditions. Such resolution reduces the possibility of wound contamination and maintenance of the injury and improves the reestablishment of tissue morphology and functions. Investigators are seeking adjuvant therapies that can accelerate wound healing and are developing new strategies for clinical applications. This study compared the effects of epidermal growth factor (EGF) application and low-level laser therapy (LLLT) on cultured epithelial cells. Cells were seeded in 24-well plates. After a 24-h incubation, the epithelial cells were either treated with EGF (100 μM in serum-free DMEM for 72 h) or subjected to LLLT (780 nm, 25 mW, 0.5, 1.5, and 3 J/cm2) by three applications every 24 h. Seventy-two hours after cells were treated with EGF or LLLT, cell migration, viability, proliferation, and collagen synthesis were assessed. Cells treated with EGF showed increased cell viability, proliferation, and collagen synthesis compared with those cells that received no treatment. LLLT enhanced cell migration; however, no significant effects of laser irradiation on other cell functions were observed. Comparison of both therapies demonstrated that EGF and LLLT enhanced specific epithelial cell activities related to wound healing.
KW - Cell migration
KW - Cell proliferation
KW - Epidermal growth factor
KW - Epithelial cells
KW - Low-level laser therapy
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U2 - 10.1007/s10103-017-2176-z
DO - 10.1007/s10103-017-2176-z
M3 - Article
C2 - 28285410
AN - SCOPUS:85014893355
SN - 0268-8921
VL - 33
SP - 445
EP - 449
JO - Lasers in Medical Science
JF - Lasers in Medical Science
IS - 2
ER -