TY - JOUR
T1 - Epitranscriptomic regulation of insecticide resistance
AU - Yang, Xin
AU - Wei, Xuegao
AU - Yang, Jing
AU - Du, Tianhua
AU - Yin, Cheng
AU - Fu, Buli
AU - Huang, Mingjiao
AU - Liang, Jinjin
AU - Gong, Peipan
AU - Liu, Shaonan
AU - Xie, Wen
AU - Guo, Zhaojiang
AU - Wang, Shaoli
AU - Wu, Qingjun
AU - Nauen, Ralf
AU - Zhou, Xuguo
AU - Bass, Chris
AU - Zhang, Youjun
N1 - Publisher Copyright:
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
PY - 2021/5/5
Y1 - 2021/5/5
N2 - N6-methyladenosine (m6A) is the most prevalent messenger RNA modification in eukaryotes and an important posttranscriptional regulator of gene expression. However, the biological roles of m6A in most insects remain largely unknown. Here, we show that m6A regulates a cytochrome P450 gene (CYP4C64) in the global whitefly pest, Bemisia tabaci, leading to insecticide resistance. Investigation of the regulation of CYP4C64, which confers resistance to the insecticide thiamethoxam, revealed a mutation in the 5′ untranslated region of this gene in resistant B. tabaci strains that introduces a predicted m6A site. We provide several lines of evidence that mRNA methylation of the adenine at this position, in combination with modified expression of m6A writers, acts to increase expression of CYP4C64 and resistance. Collectively, these results provide an example of the epitranscriptomic regulation of the xenobiotic response in insects and implicate the m6A regulatory axis in the development of insecticide resistance.
AB - N6-methyladenosine (m6A) is the most prevalent messenger RNA modification in eukaryotes and an important posttranscriptional regulator of gene expression. However, the biological roles of m6A in most insects remain largely unknown. Here, we show that m6A regulates a cytochrome P450 gene (CYP4C64) in the global whitefly pest, Bemisia tabaci, leading to insecticide resistance. Investigation of the regulation of CYP4C64, which confers resistance to the insecticide thiamethoxam, revealed a mutation in the 5′ untranslated region of this gene in resistant B. tabaci strains that introduces a predicted m6A site. We provide several lines of evidence that mRNA methylation of the adenine at this position, in combination with modified expression of m6A writers, acts to increase expression of CYP4C64 and resistance. Collectively, these results provide an example of the epitranscriptomic regulation of the xenobiotic response in insects and implicate the m6A regulatory axis in the development of insecticide resistance.
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U2 - 10.1126/sciadv.abe5903
DO - 10.1126/sciadv.abe5903
M3 - Article
C2 - 33952517
AN - SCOPUS:85105459385
VL - 7
JO - Science advances
JF - Science advances
IS - 19
M1 - eabe5903
ER -