Epitranscriptomic regulation of insecticide resistance

Xin Yang, Xuegao Wei, Jing Yang, Tianhua Du, Cheng Yin, Buli Fu, Mingjiao Huang, Jinjin Liang, Peipan Gong, Shaonan Liu, Wen Xie, Zhaojiang Guo, Shaoli Wang, Qingjun Wu, Ralf Nauen, Xuguo Zhou, Chris Bass, Youjun Zhang

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

N6-methyladenosine (m6A) is the most prevalent messenger RNA modification in eukaryotes and an important posttranscriptional regulator of gene expression. However, the biological roles of m6A in most insects remain largely unknown. Here, we show that m6A regulates a cytochrome P450 gene (CYP4C64) in the global whitefly pest, Bemisia tabaci, leading to insecticide resistance. Investigation of the regulation of CYP4C64, which confers resistance to the insecticide thiamethoxam, revealed a mutation in the 5′ untranslated region of this gene in resistant B. tabaci strains that introduces a predicted m6A site. We provide several lines of evidence that mRNA methylation of the adenine at this position, in combination with modified expression of m6A writers, acts to increase expression of CYP4C64 and resistance. Collectively, these results provide an example of the epitranscriptomic regulation of the xenobiotic response in insects and implicate the m6A regulatory axis in the development of insecticide resistance.

Original languageEnglish
Article numbereabe5903
JournalScience advances
Volume7
Issue number19
DOIs
StatePublished - May 5 2021

Bibliographical note

Publisher Copyright:
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Epitranscriptomic regulation of insecticide resistance'. Together they form a unique fingerprint.

Cite this