Equine antibody response to larval Parascaris equorum excretory-secretory products

Steffanie V. Burk; Sriveny Dangoudoubiyam; Tammy Brewster-Barnes; Daniel K. Howe; Craig N. Carter; Uneeda K. Bryant; Mary G. Rossano

doi:10.1016/j.vetpar.2016.06.036

Equine antibody response to larval Parascaris equorum excretory-secretory products

Steffanie V. Burk
, Sriveny Dangoudoubiyam
, Tammy Brewster-Barnes
, Daniel K. Howe
, Craig N. Carter
, Uneeda K. Bryant
, Mary G. Rossano

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Parascaris equorum is an intestinal nematode of foals and young horses that can produce mild to severe pathology. Current diagnosis is limited to detection of patent infections, when parasite eggs are identified during fecal examinations. This study examined the use of larval P. equorum excretory-secretory (ES) products in a western blot test for diagnosis of prepatent equine P. equorum infection. Sera from adult mares negative for patent P. equorum infections, foals prior to consuming colostrum, and P. equorum infected foals were used as controls in this study. Study samples included sera from 18 broodmares prior to parturition and sera from their foals throughout the process of natural infection. Sera from study horses were examined for IgG(T) antibody recognition of ES products. Foals naturally infected with P. equorum possessed IgG(T) antibodies against 19 kDa, 22 kDa, 26 kDa, and 34 kDa ES products. However, passive transfer of colostral antibodies from mares was shown to preclude the use of the crude larval ES product-based western blot test for diagnosis of prepatent P. equorum infections in foals.

Original language	English
Pages (from-to)	83-87
Number of pages	5
Journal	Veterinary Parasitology
Volume	226
DOIs	https://doi.org/10.1016/j.vetpar.2016.06.036
State	Published - Aug 15 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.

Funding

We would like to thank Bryan Cassill, Frank Berry, Kristin Fox, Laura Strasinger, Susan White, and Drs. Eugene Lyons, Martin Nielsen, Jacqui Matthews, Noel Inocencio, Ulla Vestergaard Andersen, Sara Mastellar, and Craig Reinemeyer for technical assistance or help with sample collection. We would also like to thank Dr. Robert Harmon and Dr. Nancy Cox of the University of Kentucky’s College of Agriculture, Food, and the Environment for their financial support of this project.

Funders
University of Kentucky’s College of Agriculture, Food

Keywords

Equine
Excretory-secretory
Horse
IgG(T)
Parascaris equorum
Western blot

ASJC Scopus subject areas

Parasitology
General Veterinary

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10.1016/j.vetpar.2016.06.036

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title = "Equine antibody response to larval Parascaris equorum excretory-secretory products",

abstract = "Parascaris equorum is an intestinal nematode of foals and young horses that can produce mild to severe pathology. Current diagnosis is limited to detection of patent infections, when parasite eggs are identified during fecal examinations. This study examined the use of larval P. equorum excretory-secretory (ES) products in a western blot test for diagnosis of prepatent equine P. equorum infection. Sera from adult mares negative for patent P. equorum infections, foals prior to consuming colostrum, and P. equorum infected foals were used as controls in this study. Study samples included sera from 18 broodmares prior to parturition and sera from their foals throughout the process of natural infection. Sera from study horses were examined for IgG(T) antibody recognition of ES products. Foals naturally infected with P. equorum possessed IgG(T) antibodies against 19 kDa, 22 kDa, 26 kDa, and 34 kDa ES products. However, passive transfer of colostral antibodies from mares was shown to preclude the use of the crude larval ES product-based western blot test for diagnosis of prepatent P. equorum infections in foals.",

keywords = "Equine, Excretory-secretory, Horse, IgG(T), Parascaris equorum, Western blot",

author = "Burk, \{Steffanie V.\} and Sriveny Dangoudoubiyam and Tammy Brewster-Barnes and Howe, \{Daniel K.\} and Carter, \{Craig N.\} and Bryant, \{Uneeda K.\} and Rossano, \{Mary G.\}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = aug,

day = "15",

doi = "10.1016/j.vetpar.2016.06.036",

language = "English",

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AU - Dangoudoubiyam, Sriveny

AU - Brewster-Barnes, Tammy

AU - Howe, Daniel K.

AU - Carter, Craig N.

AU - Bryant, Uneeda K.

AU - Rossano, Mary G.

PY - 2016/8/15

Y1 - 2016/8/15

N2 - Parascaris equorum is an intestinal nematode of foals and young horses that can produce mild to severe pathology. Current diagnosis is limited to detection of patent infections, when parasite eggs are identified during fecal examinations. This study examined the use of larval P. equorum excretory-secretory (ES) products in a western blot test for diagnosis of prepatent equine P. equorum infection. Sera from adult mares negative for patent P. equorum infections, foals prior to consuming colostrum, and P. equorum infected foals were used as controls in this study. Study samples included sera from 18 broodmares prior to parturition and sera from their foals throughout the process of natural infection. Sera from study horses were examined for IgG(T) antibody recognition of ES products. Foals naturally infected with P. equorum possessed IgG(T) antibodies against 19 kDa, 22 kDa, 26 kDa, and 34 kDa ES products. However, passive transfer of colostral antibodies from mares was shown to preclude the use of the crude larval ES product-based western blot test for diagnosis of prepatent P. equorum infections in foals.

AB - Parascaris equorum is an intestinal nematode of foals and young horses that can produce mild to severe pathology. Current diagnosis is limited to detection of patent infections, when parasite eggs are identified during fecal examinations. This study examined the use of larval P. equorum excretory-secretory (ES) products in a western blot test for diagnosis of prepatent equine P. equorum infection. Sera from adult mares negative for patent P. equorum infections, foals prior to consuming colostrum, and P. equorum infected foals were used as controls in this study. Study samples included sera from 18 broodmares prior to parturition and sera from their foals throughout the process of natural infection. Sera from study horses were examined for IgG(T) antibody recognition of ES products. Foals naturally infected with P. equorum possessed IgG(T) antibodies against 19 kDa, 22 kDa, 26 kDa, and 34 kDa ES products. However, passive transfer of colostral antibodies from mares was shown to preclude the use of the crude larval ES product-based western blot test for diagnosis of prepatent P. equorum infections in foals.

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KW - Excretory-secretory

KW - Horse

KW - IgG(T)

KW - Parascaris equorum

KW - Western blot

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ER -

Equine antibody response to larval Parascaris equorum excretory-secretory products

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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