Equine herpesvirus-1 (EHV-1) is one of the most common and important respiratory viral pathogens of horses. EHV-1 in horses replicates initially in the respiratory epithelium and then spreads systematically to endothelial cells lining the small blood vessels in the uterus and spinal cord, and highly pathogenic virus strains can produce aborted fetuses or myeloencephalopathy. Like other herpes viruses, EHV-1 employs a variety of mechanisms for immune evasion. Some herpes viruses down-regulate the type-I interferon (IFN) response to infection, but such activity has not been described for EHV-1. Here, in an in vitro system utilizing an established equine endothelial cell line, we studied the temporal effect on IFN-β responses following infection with the neuropathogenic T953 strain of EHV-1. Results show that after an early induction of IFN-β, the virus actively shut down further production of IFN-β and this was correlated with expression of the viral late genes. Expression of the IFN response factor viperin, a marker of host cell type-I IFN responses, was also suppressed by T953 virus infection. EHV-1-mediated suppression of host type-I IFN responses may play an important role in EHV-1 pathogenesis and the mechanism of this, presumably involving a viral late gene product, warrants investigation.
|Number of pages||8|
|Journal||Veterinary Immunology and Immunopathology|
|State||Published - Oct 15 2015|
Bibliographical noteFunding Information:
We wish to acknowledge the generous technical assistance of Ms. Stephanie Reedy. We thank Dr. Adolfo Garcia-Sastre (Mount Sinai School of Medicine, New York) and Dr. Dennis O’Callaghan (Louisiana State University) for provision of reagents. This work was supported by grants from the American Quarter Horse Foundation and the Grayson Jockey Club Research Foundation . Sanjay Sarkar was supported by a fellowship from the Geoffrey C. Hughes Foundation . The work is part of a project of the Kentucky Agricultural Experiment Station ( KY014041 ) and is published with the approval of the Director (publication no. 15-14-077).
© 2015 Elsevier B.V.
- Immune evasion
- Innate immunity
ASJC Scopus subject areas
- Veterinary (all)