Equine infl uenza culture methods

Thomas M. Chambers; Stephanie E. Reedy

doi:10.1007/978-1-4939-0758-8_35

Equine infl uenza culture methods

Thomas M. Chambers, Stephanie E. Reedy

Veterinary Science

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate effi ciently in 11-day-old eggs, but we fi nd that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

Original language	English
Pages (from-to)	403-410
Number of pages	8
Journal	Methods in Molecular Biology
Volume	1161
DOIs	https://doi.org/10.1007/978-1-4939-0758-8_35
State	Published - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.

Keywords

Embryonated eggs
MDCK
Mammalian infl uenza
Plaque assay
TCID assay

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-0758-8_35

Cite this

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title = "Equine infl uenza culture methods",

abstract = "Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate effi ciently in 11-day-old eggs, but we fi nd that equine-1 viruses kill the embryos whereas equine-2 viruses do not.",

keywords = "Embryonated eggs, MDCK, Mammalian infl uenza, Plaque assay, TCID assay",

author = "Chambers, {Thomas M.} and Reedy, {Stephanie E.}",

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year = "2014",

doi = "10.1007/978-1-4939-0758-8_35",

language = "English",

volume = "1161",

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AU - Reedy, Stephanie E.

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AB - Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate effi ciently in 11-day-old eggs, but we fi nd that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

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KW - MDCK

KW - Mammalian infl uenza

KW - Plaque assay

KW - TCID assay

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Equine infl uenza culture methods

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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