Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate effi ciently in 11-day-old eggs, but we fi nd that equine-1 viruses kill the embryos whereas equine-2 viruses do not.
|Number of pages||8|
|Journal||Methods in Molecular Biology|
|State||Published - 2014|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media New York 2014.
- Embryonated eggs
- Mammalian infl uenza
- Plaque assay
- TCID assay
ASJC Scopus subject areas
- Molecular Biology