Equine infl uenza culture methods

Thomas M. Chambers, Stephanie E. Reedy

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate effi ciently in 11-day-old eggs, but we fi nd that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

Original languageEnglish
Pages (from-to)403-410
Number of pages8
JournalMethods in Molecular Biology
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.


  • Embryonated eggs
  • MDCK
  • Mammalian infl uenza
  • Plaque assay
  • TCID assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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