Equine infl uenza culture methods

Thomas M. Chambers, Stephanie E. Reedy

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate effi ciently in 11-day-old eggs, but we fi nd that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

Original languageEnglish
Pages (from-to)403-410
Number of pages8
JournalMethods in Molecular Biology
Volume1161
DOIs
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.

Keywords

  • Embryonated eggs
  • MDCK
  • Mammalian infl uenza
  • Plaque assay
  • TCID assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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