Abstract
Equine influenza viruses are cultured in embryonated hen eggs, or in mammalian cells, generally Madin–Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate effi ciently in 11-day-old eggs, but we fi nd that equine-1 viruses kill the embryos whereas equine-2 viruses do not.
Original language | English |
---|---|
Pages (from-to) | 403-410 |
Number of pages | 8 |
Journal | Methods in Molecular Biology |
Volume | 1161 |
DOIs | |
State | Published - 2014 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2014.
Keywords
- Embryonated eggs
- MDCK
- Mammalian infl uenza
- Plaque assay
- TCID assay
ASJC Scopus subject areas
- Molecular Biology
- Genetics