Abstract
Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Pages | 393-400 |
Number of pages | 8 |
DOIs | |
State | Published - 2020 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2123 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020.
Keywords
- Embryonated eggs
- MDCK
- Mammalian influenza
- Plaque assay
- TCID assay
ASJC Scopus subject areas
- Molecular Biology
- Genetics