Equine influenza culture methods

Thomas M. Chambers; Stephanie E. Reedy

doi:10.1007/978-1-0716-0346-8_30

Equine influenza culture methods

Thomas M. Chambers, Stephanie E. Reedy

Veterinary Science

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	393-400
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-0716-0346-8_30
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2123
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020.

Keywords

Embryonated eggs
MDCK
Mammalian influenza
Plaque assay
TCID assay

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-0346-8_30

Cite this

@inbook{0b1d404579b441e1a8bad48a3c231b1a,

title = "Equine influenza culture methods",

abstract = "Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.",

keywords = "Embryonated eggs, MDCK, Mammalian influenza, Plaque assay, TCID assay",

author = "Chambers, {Thomas M.} and Reedy, {Stephanie E.}",

note = "Publisher Copyright: {\textcopyright} This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020.",

year = "2020",

doi = "10.1007/978-1-0716-0346-8_30",

language = "English",

series = "Methods in Molecular Biology",

pages = "393--400",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Equine influenza culture methods

AU - Chambers, Thomas M.

AU - Reedy, Stephanie E.

N1 - Publisher Copyright: © This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020.

PY - 2020

Y1 - 2020

N2 - Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

AB - Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

KW - Embryonated eggs

KW - MDCK

KW - Mammalian influenza

KW - Plaque assay

KW - TCID assay

UR - http://www.scopus.com/inward/record.url?scp=85082098042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85082098042&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-0346-8_30

DO - 10.1007/978-1-0716-0346-8_30

M3 - Chapter

C2 - 32170705

AN - SCOPUS:85082098042

T3 - Methods in Molecular Biology

SP - 393

EP - 400

BT - Methods in Molecular Biology

ER -

Equine influenza culture methods

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this