Equine influenza culture methods

Thomas M. Chambers, Stephanie E. Reedy

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages8
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020.


  • Embryonated eggs
  • MDCK
  • Mammalian influenza
  • Plaque assay
  • TCID assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Equine influenza culture methods'. Together they form a unique fingerprint.

Cite this