Equine influenza serological methods

Thomas M. Chambers, Stephanie E. Reedy

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Serologic tests for equine infl uenza virus (EIV) antibodies are used for many purposes, including retrospective diagnosis, subtyping of virus isolates, antigenic comparison of different virus strains, and measurement of immune responses to EIV vaccines. The hemagglutination-inhibition (HI), single radial hemolysis (SRH), and serum micro-neutralization tests are the most widely used for these purposes and are described here. The presence of inhibitors of hemagglutination in equine serum complicates interpretation of HI assay results, and there are alternative protocols (receptor-destroying enzyme, periodate, trypsin- periodate) for their removal. With the EIV H3N8 strains in particular, equine antibody titers may be magnified by pretreating the HI test antigen with Tween-80 and ether. The SRH assay offers stronger correlations between serum antibody titers and protection from disease. Other tests are sometimes used for specialized purposes such as the neuraminidase-inhibition assay for subtyping, or ELISA for measuring different specific antibody isotypes, and are not described here.

Original languageEnglish
Pages (from-to)411-422
Number of pages12
JournalMethods in Molecular Biology
StatePublished - 2014

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2014.


  • Antibody
  • Hemagglutination inhibition
  • Hemolysis
  • Influenza
  • Neutralization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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