ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

Xianglin Shi; N. S. Dalal

doi:10.3109/10715769009145929

ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

Xianglin Shi, N. S. Dalal

Toxicology and Cancer Biology

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H₂O₂) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H₂O₂ to generate the OH radicals. Reaction between H₂O₂ and a Cr(V)-glutathione complex. and between H₂O₂ and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H₂O₂, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.

Original language	English
Pages (from-to)	17-26
Number of pages	10
Journal	Free Radical Research
Volume	10
Issue number	1-2
DOIs	https://doi.org/10.3109/10715769009145929
State	Published - 1990

Bibliographical note

Funding Information:
This research has been supported by the Department of the Interior’s Mineral program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant

Keywords

Cr(V)
Cr(VI) reduction
ESR
Free radicals
Hydrogen peroxide
Spin trapping

ASJC Scopus subject areas

Biochemistry

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@article{93739839703c4b26a0ca0158dfadd1cc,

title = "ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide",

abstract = "Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.",

keywords = "Cr(V), Cr(VI) reduction, ESR, Free radicals, Hydrogen peroxide, Spin trapping",

author = "Xianglin Shi and Dalal, {N. S.}",

note = "Funding Information: This research has been supported by the Department of the Interior{\textquoteright}s Mineral program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant",

year = "1990",

doi = "10.3109/10715769009145929",

language = "English",

volume = "10",

pages = "17--26",

number = "1-2",

}

TY - JOUR

T1 - ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

AU - Shi, Xianglin

AU - Dalal, N. S.

N1 - Funding Information: This research has been supported by the Department of the Interior’s Mineral program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under grant

PY - 1990

Y1 - 1990

N2 - Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.

AB - Electron spin resonance (ESR) measurments provide direct evidence for the involvement of Cr(V) in the reduction of Cr(VI) by NAD(P)H. Addition of hydrogen peroxide (H2O2) to NAD(P)H-Cr(VI) reaction mixtures suppresses the Cr(V) signal and generates hydroxyl (OH) radicals (as detected via spin trapping), suggesting that Cr(V) reacts with H2O2 to generate the OH radicals. Reaction between H2O2 and a Cr(V)-glutathione complex. and between H2O2 and several Cr(V)-cdrboxylato complexes also produces OH radicals. These results suggest that Cr(V) complexes catalyze the generation of OH radicals from H2O2, and that OH radicals might play a significant role in the mechanism of Cr(VI) cytotoxicity.

KW - Cr(V)

KW - Cr(VI) reduction

KW - ESR

KW - Free radicals

KW - Hydrogen peroxide

KW - Spin trapping

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U2 - 10.3109/10715769009145929

DO - 10.3109/10715769009145929

M3 - Article

C2 - 2165982

AN - SCOPUS:0025653302

SN - 1071-5762

VL - 10

SP - 17

EP - 26

JO - Free Radical Research

JF - Free Radical Research

IS - 1-2

ER -

ESR spin trapping detection of hydroxyl radicals in the reactions of cr(v) complexes with hydrogen peroxide

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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