ESR spin trapping investigation on peroxynitrite decomposition: No evidence for hydroxyl radical production

Xianglin L. Shi, Amy Lenhart, Yan Mao

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The decomposition of peroxynitrite in the presence of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) generated 5,5-dimethylpyrrolidone-(2)-oxy-(1) (DMPOX) without formation of DMPO/OH. Formate enhanced the peroxynitrite decomposition but did not generate any detectable amount of formate-derived free radicals. Glutathione, cysteine, penicillamine, and ascorbate reacted with pyroxynitrite to generate the corresponding thiyl and ascorbyl radicals. The results show that the decomposition of peroxynitrite did not generate any significant amount of OH radicals, and one-electron reduction of peroxynitrite by ascorbate may be one of the important peroxynitrite detoxification pathways.

Original languageEnglish
Pages (from-to)1515-1521
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number3
DOIs
StatePublished - Sep 30 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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