Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.
|BioMed Research International
|Published - 2018
Bibliographical noteFunding Information:
The authors thank Dr. Ping Jiang for providing the PRRSV S1 and SY0608 strain. This work was supported by the National Key Research and Development Program of China (2016YFD0501505), Funds for Distinguished Young Scientists of Shandong Province (JQ201411), Young Taishan Scholars (tsqn20161057) and Distinguished Taishan Scholars, Key Project of Scientific and Technological Innovation of Shandong Academy of Agricultural Sciences (2014CXZ08-2), Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2016C07), Natural Science Foundation of Shandong Province (ZR2014YL013), and the National Natural Science Foundation of China (31370189, 31672609, 31602089, and 31502051).
© 2018 Xiangju Wu et al.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (all)
- Immunology and Microbiology (all)