Establishment of an in vitro method for evaluating whitefly resistance in tomato

Guangjun Guo, Jianchang Gao, Xiaoxuan Wang, Yanmei Guo, J. C. Snyder, Yongchen Du

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


An accurate and simple evaluation method is crucial for identifying whitefly resistance in tomato breeding. We developed an in vitro method for evaluating resistance of tomato leaves and tested this on wild and cultivated tomato varieties. We found that young leaves observed for whitefly oviposition after 8 hours provided appropriate comparative conditions. This method effectively distinguished resistance among tomato cultivars and wild species and also demonstrated significant difference in oviposition rates among leaf positions on susceptible cultivars. The in vitro test was as precise as in vivo test using intact plants and had advantages over in vivo test, and can be used for evaluating resistance in large populations.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalBreeding Science
Issue number3
StatePublished - Oct 8 2013


  • Bemisia tabaci
  • Breeding
  • Phenotypic testing
  • Solanum habrochaites
  • Solanum lycopersicum
  • Tomato resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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