Abstract
Cellular esterase activity and the ability to exclude propidium iodide were examined after exposing tumor cells to anticancer agents. In general, esterase activity and the ability to exclude propidium iodide continued when cells proliferated and disappeared when proliferation was inhibited. However, with a number of preparations, drug exposure inhibited proliferation while esterase activity and propidium iodide exclusion persisted. These indications of persisting cell function or viability after drug exposure may be relevant to a potential for tumor cell recovery. When the viability of established cell lines progressively declined on days 4 and 7 following drug exposure, recovery did not occur. When proliferative recoveries occurred, viabilities remained elevated. Estimates of in vitro sensitivity by proliferation-related criteria were contrasted by persistent high viability estimates in 22% of the determinations performed with primary tumor cell preparations. The potential for recovery may explain the disappointing ability of proliferative chemosensitivity assays to predict clinical sensitivity.
| Original language | English |
|---|---|
| Pages (from-to) | 413-426 |
| Number of pages | 14 |
| Journal | Cancer Investigation |
| Volume | 3 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1985 |
Bibliographical note
Funding Information:script preparation and the a\sistance of Cay Ramey Lane with graphics. This work was supported by the Vetcrans Administration. Certain agents used in this work were obtained from the Drug Synthesis and Chemistry Branch, Division of Cancer Treatment, NCI. while others were provided by the Natural Products Branch. Division of Cancer Treatment. NCI. A preliminary presentation of this work uas made at the 74th Annual Meeting of the American Aswciation for Cancer Research, San Diego. California (May 1983).
ASJC Scopus subject areas
- Oncology
- Cancer Research
