Estradiol-regulated proline-rich acid protein 1 is repressed by class I histone deacetylase and functions in peri-implantation mouse uterus

Gao Feng Xiong, Yan Shuai Zhang, Bing Chen Han, Wen Chen, Ying Yang, Jing Pian Peng

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Secretory protein proline-rich acid protein 1 (PRAP1) is abundantly expressed in late pregnant uterus. However, regulation and function of PRAP1 in pregnant uterus is still elusive. We firstly reported differential expression of PRAP1 in peri-implantation uteri and its localization in endometrial epithelia. Expression of PRAP1 in uterus was induced by 17β-estradiol and its expression showed a negative correlation with that of class Ihistone deacetylases (HDACs) in isolated endometrial epithelia. PRAP1 was increased by HDACs inhibitor sodium butyrate treatment, while decreased significantly by estrogen receptor inhibitor ICI182,780 via up-regulating class IHDACs. Number of implanted embryos was decreased in mice immunized with pCR3.1-PRAP1 or injected with rabbit anti-PRAP1 antibody. DNA immunization or antibody injection could affect apoptosis and expression of cytokines (IL-4, IFN-γ). In conclusion, both 17β-estradiol and class IHDACs are involved in modulating PRAP1 expression in peri-implantation uteri. Preliminary functional research indicates that neutralizing PRAP1 protein causes reduction of implanted embryos.

Original languageEnglish
Pages (from-to)23-33
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume331
Issue number1
DOIs
StatePublished - Jan 1 2011

Bibliographical note

Funding Information:
The authors are grateful to Prof. Qing-Yuan Sun for his revision on writing. This work was supported by the grants from “ National Basic Research Program of China ” ( 2006CB944007 ; 2006CB504006 ), “the National Natural Science Foundation of China ” ( 30770246 ), “ Beijing Natural Science Foundation ” ( 5071001 ).

Keywords

  • 17β-Estradiol
  • Endometrial epithelia
  • HDACs
  • PRAP1
  • Peri-implantation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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