Ethanol inhibits the uptake of exogenous norepinephrine from the extracellular space of the rat cerebellum

Anya M.Y. Lin; Paula C. Bickford; Michael R. Palmer; Greg A. Gerhardt

doi:10.1016/0304-3940(93)90860-N

Ethanol inhibits the uptake of exogenous norepinephrine from the extracellular space of the rat cerebellum

Anya M.Y. Lin, Paula C. Bickford, Michael R. Palmer, Greg A. Gerhardt

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Rapid chronoamperometric recordings using nafion-coated carbon fiber electrodes coupled with pressure-ejection of drugs were used to investigate the effects of ethanol on norepinephrine (NE)-containing nerve terminals in the urethane-anesthetized Fischer 344 rat. Local application of ethanol from a double-barrel micropipette did not produce detectable changes in extracellular levels of NE in the rat cerebellar cortex. However, when ethanol was applied prior to local application of NE, it was seen to inhibit the uptake of NE from the extracellular space. These results were compared to the effects seen from the local application of a known high-affinity uptake inhibitor, nomifensine. Nomifensine was found to inhibit the extracellular uptake of NE in rat cerebeller cortex similar to ethanol. Our results support the hypothesis that one effect of ethanol on the noradrenergic system of the rat cerebellum is an alteration in the uptake of NE into NE-containing nerve endings. In addition, the present data concerning ethanol-induced inhibition of NE clearance or uptake support our previous electrophysiological studies in which we found that ethanol can potentiate the modulatory effects of β-agonists on GABA responses of cerebellar Purkinje neurons.

Original language	English
Pages (from-to)	71-75
Number of pages	5
Journal	Neuroscience Letters
Volume	164
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(93)90860-N
State	Published - Dec 24 1993

Bibliographical note

Funding Information:
The authors thank David Young for his help with statistical analysis, and special thanks to Laura Lee Lamothe for expert typing and processing of this manuscript. This work was supported by USPHS Grants AG06434, AG00441, AA05915, AA00102 and AG04418, the Veteran Administration Medical Research Services, and NSF BNS-9110308. Michael R. Palmer is supported by an ADAMHA Research Scientist Development Award. A. M.-Y. Lin is supported by an Educational Grant 27051 F from National Science Council and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.

Funding

The authors thank David Young for his help with statistical analysis, and special thanks to Laura Lee Lamothe for expert typing and processing of this manuscript. This work was supported by USPHS Grants AG06434, AG00441, AA05915, AA00102 and AG04418, the Veteran Administration Medical Research Services, and NSF BNS-9110308. Michael R. Palmer is supported by an ADAMHA Research Scientist Development Award. A. M.-Y. Lin is supported by an Educational Grant 27051 F from National Science Council and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.

Funders	Funder number
ADAMHA	27051 F
Academia Sinica, Institute of Biomedical Sciences
United States Department of Veteran Administration
National Science Foundation (NSF)	BNS-9110308
National Institute on Alcohol Abuse and Alcoholism	R01AA005915
U.S. Public Health Service	AG00441, AG06434, AA05915, AA00102, AG04418
National Science Council
Academia Sinica, Institute of Chemistry

Keywords

Cerebellar cortex
Ethanol
Fischer 344 rat
High-affinity catecholamine uptake
In vivo electrochemistry
Norepinephrine

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0304-3940(93)90860-N

Cite this

@article{5a55007dbb3847b6a1fb8e0d469c07c8,

title = "Ethanol inhibits the uptake of exogenous norepinephrine from the extracellular space of the rat cerebellum",

abstract = "Rapid chronoamperometric recordings using nafion-coated carbon fiber electrodes coupled with pressure-ejection of drugs were used to investigate the effects of ethanol on norepinephrine (NE)-containing nerve terminals in the urethane-anesthetized Fischer 344 rat. Local application of ethanol from a double-barrel micropipette did not produce detectable changes in extracellular levels of NE in the rat cerebellar cortex. However, when ethanol was applied prior to local application of NE, it was seen to inhibit the uptake of NE from the extracellular space. These results were compared to the effects seen from the local application of a known high-affinity uptake inhibitor, nomifensine. Nomifensine was found to inhibit the extracellular uptake of NE in rat cerebeller cortex similar to ethanol. Our results support the hypothesis that one effect of ethanol on the noradrenergic system of the rat cerebellum is an alteration in the uptake of NE into NE-containing nerve endings. In addition, the present data concerning ethanol-induced inhibition of NE clearance or uptake support our previous electrophysiological studies in which we found that ethanol can potentiate the modulatory effects of β-agonists on GABA responses of cerebellar Purkinje neurons.",

keywords = "Cerebellar cortex, Ethanol, Fischer 344 rat, High-affinity catecholamine uptake, In vivo electrochemistry, Norepinephrine",

author = "Lin, {Anya M.Y.} and Bickford, {Paula C.} and Palmer, {Michael R.} and Gerhardt, {Greg A.}",

note = "Funding Information: The authors thank David Young for his help with statistical analysis, and special thanks to Laura Lee Lamothe for expert typing and processing of this manuscript. This work was supported by USPHS Grants AG06434, AG00441, AA05915, AA00102 and AG04418, the Veteran Administration Medical Research Services, and NSF BNS-9110308. Michael R. Palmer is supported by an ADAMHA Research Scientist Development Award. A. M.-Y. Lin is supported by an Educational Grant 27051 F from National Science Council and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.",

year = "1993",

month = dec,

day = "24",

doi = "10.1016/0304-3940(93)90860-N",

language = "English",

volume = "164",

pages = "71--75",

number = "1-2",

}

TY - JOUR

T1 - Ethanol inhibits the uptake of exogenous norepinephrine from the extracellular space of the rat cerebellum

AU - Lin, Anya M.Y.

AU - Bickford, Paula C.

AU - Palmer, Michael R.

AU - Gerhardt, Greg A.

N1 - Funding Information: The authors thank David Young for his help with statistical analysis, and special thanks to Laura Lee Lamothe for expert typing and processing of this manuscript. This work was supported by USPHS Grants AG06434, AG00441, AA05915, AA00102 and AG04418, the Veteran Administration Medical Research Services, and NSF BNS-9110308. Michael R. Palmer is supported by an ADAMHA Research Scientist Development Award. A. M.-Y. Lin is supported by an Educational Grant 27051 F from National Science Council and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.

PY - 1993/12/24

Y1 - 1993/12/24

N2 - Rapid chronoamperometric recordings using nafion-coated carbon fiber electrodes coupled with pressure-ejection of drugs were used to investigate the effects of ethanol on norepinephrine (NE)-containing nerve terminals in the urethane-anesthetized Fischer 344 rat. Local application of ethanol from a double-barrel micropipette did not produce detectable changes in extracellular levels of NE in the rat cerebellar cortex. However, when ethanol was applied prior to local application of NE, it was seen to inhibit the uptake of NE from the extracellular space. These results were compared to the effects seen from the local application of a known high-affinity uptake inhibitor, nomifensine. Nomifensine was found to inhibit the extracellular uptake of NE in rat cerebeller cortex similar to ethanol. Our results support the hypothesis that one effect of ethanol on the noradrenergic system of the rat cerebellum is an alteration in the uptake of NE into NE-containing nerve endings. In addition, the present data concerning ethanol-induced inhibition of NE clearance or uptake support our previous electrophysiological studies in which we found that ethanol can potentiate the modulatory effects of β-agonists on GABA responses of cerebellar Purkinje neurons.

AB - Rapid chronoamperometric recordings using nafion-coated carbon fiber electrodes coupled with pressure-ejection of drugs were used to investigate the effects of ethanol on norepinephrine (NE)-containing nerve terminals in the urethane-anesthetized Fischer 344 rat. Local application of ethanol from a double-barrel micropipette did not produce detectable changes in extracellular levels of NE in the rat cerebellar cortex. However, when ethanol was applied prior to local application of NE, it was seen to inhibit the uptake of NE from the extracellular space. These results were compared to the effects seen from the local application of a known high-affinity uptake inhibitor, nomifensine. Nomifensine was found to inhibit the extracellular uptake of NE in rat cerebeller cortex similar to ethanol. Our results support the hypothesis that one effect of ethanol on the noradrenergic system of the rat cerebellum is an alteration in the uptake of NE into NE-containing nerve endings. In addition, the present data concerning ethanol-induced inhibition of NE clearance or uptake support our previous electrophysiological studies in which we found that ethanol can potentiate the modulatory effects of β-agonists on GABA responses of cerebellar Purkinje neurons.

KW - Cerebellar cortex

KW - Ethanol

KW - Fischer 344 rat

KW - High-affinity catecholamine uptake

KW - In vivo electrochemistry

KW - Norepinephrine

UR - http://www.scopus.com/inward/record.url?scp=0027738091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027738091&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(93)90860-N

DO - 10.1016/0304-3940(93)90860-N

M3 - Article

C2 - 8152619

AN - SCOPUS:0027738091

SN - 0304-3940

VL - 164

SP - 71

EP - 75

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Ethanol inhibits the uptake of exogenous norepinephrine from the extracellular space of the rat cerebellum

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this