Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

Luzia Aparecida Costa Barreira; Priscila Santos Scheucher; Marilia Farignoli Romeiro; Leonardo La Serra; Soraya Jabur Badra; William Marciel De Souza; Luiz Tadeu Moraes Figueiredo

doi:10.1590/0037-8682-0404-2017

Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

Luzia Aparecida Costa Barreira
, Priscila Santos Scheucher
, Marilia Farignoli Romeiro
, Leonardo La Serra
, Soraya Jabur Badra
, William Marciel De Souza
, Luiz Tadeu Moraes Figueiredo

Microbiology, Immunology, and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). Results: All three assays identified 29.1% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.

Original language	English
Pages (from-to)	168-173
Number of pages	6
Journal	Revista da Sociedade Brasileira de Medicina Tropical
Volume	51
Issue number	2
DOIs	https://doi.org/10.1590/0037-8682-0404-2017
State	Published - Mar 1 2018

Bibliographical note

Publisher Copyright:
© 2018, Sociedade Brasileira de Medicina Tropical. All rights reserved.

Funding

This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Brazil (Grant number 14/02438-6 and Scholarship numbers 12/24150-9, 17/13981-0 and 14/20851-8).

Funders	Funder number
Fundação de Amparo à Pesquisa do Estado de São Paulo	17/13981-0, 12/24150-9, 14/02438-6, 14/20851-8

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Arbovirus
Dengue virus
Flavivirus
Flow cytometry
Viral diagnostic

ASJC Scopus subject areas

Parasitology
Microbiology (medical)
Infectious Diseases

Access to Document

10.1590/0037-8682-0404-2017

Cite this

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title = "Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells",

abstract = "Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). Results: All three assays identified 29.1\% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1\% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.",

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Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells. / Barreira, Luzia Aparecida Costa; Scheucher, Priscila Santos; Romeiro, Marilia Farignoli et al.
In: Revista da Sociedade Brasileira de Medicina Tropical, Vol. 51, No. 2, 01.03.2018, p. 168-173.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

AU - Barreira, Luzia Aparecida Costa

AU - Scheucher, Priscila Santos

AU - Romeiro, Marilia Farignoli

AU - La Serra, Leonardo

AU - Badra, Soraya Jabur

AU - De Souza, William Marciel

AU - Figueiredo, Luiz Tadeu Moraes

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). Results: All three assays identified 29.1% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.

AB - Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). Results: All three assays identified 29.1% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.

KW - Arbovirus

KW - Dengue virus

KW - Flavivirus

KW - Flow cytometry

KW - Viral diagnostic

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Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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