Evaluation of Cell Wall Chemistry of della and Its Mutant Sweet Sorghum Stalks

Endalkachew Mengistie; Abdulbaset M. Alayat; Farid Sotoudehnia; Norbert Bokros; Seth DeBolt; Armando G. McDonald

doi:10.1021/acs.jafc.1c07176

Evaluation of Cell Wall Chemistry of della and Its Mutant Sweet Sorghum Stalks

Endalkachew Mengistie, Abdulbaset M. Alayat, Farid Sotoudehnia, Norbert Bokros, Seth DeBolt, Armando G. McDonald

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3 Scopus citations

Abstract

The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR). The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons. In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively. The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N. The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes. Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected. The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin. These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.

Original language	English
Pages (from-to)	1689-1703
Number of pages	15
Journal	Journal of Agricultural and Food Chemistry
Volume	70
Issue number	5
DOIs	https://doi.org/10.1021/acs.jafc.1c07176
State	Published - Feb 9 2022

Bibliographical note

Publisher Copyright:
© 2022 American Chemical Society.

Funding

The authors would like to acknowledge (i) the financial support from the National Science Foundation, Grant # 1826715, (ii) the University of Idaho Equipment and Infrastructure Support (EIS) Awards Program from the Office of Research and Economic Development (ORED) RISE Funding Program and the College of Natural Resources for supporting the purchase of the GCMS, (iii) Dr. Thomas Williams for performing the XRD analyses, and (iv) The M. J. Murdock Charitable Trust, Vancouver, WA, Reference No.: 2014120:MNL:11/20/2014, for supporting the purchase of the NMR spectrometer.

Funders	Funder number
Division of Community and Natural Resources Community College	2014120, MNL:11/20/2014
University of Idaho Equipment and Infrastructure Support
National Science Foundation (NSF)	1826715
Office of Research and Economic Development, Mississippi State University
Office of Research and Economic Development, Louisiana State University
English Institute of Sport

Keywords

2D-NMR
cellulose
internodes
lignin
nodes
sorghum

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/acs.jafc.1c07176

Cite this

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title = "Evaluation of Cell Wall Chemistry of della and Its Mutant Sweet Sorghum Stalks",

abstract = "The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR). The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons. In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively. The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N. The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes. Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected. The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin. These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.",

keywords = "2D-NMR, cellulose, internodes, lignin, nodes, sorghum",

author = "Endalkachew Mengistie and Alayat, {Abdulbaset M.} and Farid Sotoudehnia and Norbert Bokros and Seth DeBolt and McDonald, {Armando G.}",

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language = "English",

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AU - Mengistie, Endalkachew

AU - Alayat, Abdulbaset M.

AU - Sotoudehnia, Farid

AU - Bokros, Norbert

AU - DeBolt, Seth

AU - McDonald, Armando G.

PY - 2022/2/9

Y1 - 2022/2/9

N2 - The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR). The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons. In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively. The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N. The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes. Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected. The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin. These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.

AB - The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR). The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons. In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively. The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N. The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes. Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected. The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin. These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.

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Evaluation of Cell Wall Chemistry of della and Its Mutant Sweet Sorghum Stalks

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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