Evaluation of immune responses following infection of ponies with an EHV-1 ORF1/2 deletion mutant

Gisela Soboll Hussey, Stephen B. Hussey, Bettina Wagner, David W. Horohov, Gerlinde R. Van De Walle, Nikolaus Osterrieder, Lutz S. Goehring, Sangeeta Rao, David P. Lunn

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Equine herpesvirus-1 (EHV-1) infection remains a significant problem despite the widespread use of vaccines. The inability to generate a protective immune response to EHV-1 vaccination or infection is thought to be due to immunomodulatory properties of the virus, and the ORF1 and ORF2 gene products have been hypothesized as potential candidates with immunoregulatory properties. A pony infection study was performed to define immune responses to EHV-1, and to determine if an EHV-1 ORF1/2 deletion mutant (ORF1/2) would have different disease and immunoregulatory effects compared to wild type EHV-1 (WT). Infection with either virus led to cytokine responses that coincided with the course of clinical disease, particularly the biphasic pyrexia, which correlates with respiratory disease and viremia, respectively. Similarly, both viruses caused suppression of proliferative T-cell responses on day 7 post infection (pi). The ORF1/ORF2 virus caused significantly shorter primary pyrexia and significantly reduced nasal shedding, and an attenuated decrease in PBMC IL-8 as well as increased Tbet responses compared to WT-infected ponies. In conclusion, our findings are (i) that infection of ponies with EHV-1 leads to modulation of immune responses, which are correlated with disease pathogenesis, and (ii) that the ORF1/2 genes are of importance for disease outcome and modulation of cytokine responses.

Original languageEnglish
Article number23
JournalVeterinary Research
Volume42
Issue number1
DOIs
StatePublished - 2011

Bibliographical note

Funding Information:
This study was supported by grants from the Grayson-Jockey Club Research Foundation, the USDA and the College Research Council Foundation at CSU.

ASJC Scopus subject areas

  • General Medicine

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