Evaluation of inhibitor of apoptosis genes as targets for RNAi-mediated control of insect pests

June Sun Yoon; Jinmo Koo; Smitha George; Subba R. Palli

doi:10.1002/arch.21689

Evaluation of inhibitor of apoptosis genes as targets for RNAi-mediated control of insect pests

June Sun Yoon
, Jinmo Koo
, Smitha George
, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Apoptosis has been widely studied from mammals to insects. Inhibitor of apoptosis (IAP) protein is a negative regulator of apoptosis. Recent studies suggest that iap genes could be excellent targets for RNA interference (RNAi)-mediated control of insect pests. However, not much is known about iap genes in one of the well-known insect model species, Tribolium castaneum. The orthologues of five iap genes were identified in T. castaneum by searching its genome at NCBI (https://www.ncbi.nlm.nih.gov/) and UniProt (https://www.uniprot.org/) databases using Drosophila melanogaster and Aedes aegypti IAP protein sequences as queries. RNAi assays were performed in T. castaneum cell line (TcA) and larvae. The knockdown of iap1 gene induced a distinct apoptotic phenotype in TcA cells and induced 91% mortality in T. castaneum larvae. Whereas, knockdown of iap5 resulted in a decrease in cell proliferation in TcA cells and developmental defects in T. castaneum larvae which led to 100% mortality. Knockdown of the other three iap genes identified did not cause a significant effect on cells or insects. These data increase our understanding of iap genes in insects and provide opportunities for developing iap1 and iap5 as targets for RNAi-based insect pest control.

Original language	English
Article number	e21689
Journal	Archives of Insect Biochemistry and Physiology
Volume	104
Issue number	4
DOIs	https://doi.org/10.1002/arch.21689
State	Published - Aug 1 2020

Bibliographical note

Publisher Copyright:
© 2020 Wiley Periodicals LLC

Funding

Supported by grants from the National Institutes of Health (GM070559‐14 and 1R21AI131427‐01), the National Science Foundation (Industry/University Cooperative Research Centers, the Center for Arthropod Management Technologies under Grant IIP‐1821936), and the National Institute of Food and Agriculture, US Department of Agriculture (under HATCH Project 2353057000).

Funders	Funder number
National Science Foundation Arctic Social Science Program
National Institutes of Health (NIH)	GM070559‐14
National Institutes of Health (NIH)
National Institute of Allergy and Infectious Diseases	R21AI131427
National Institute of Allergy and Infectious Diseases
U.S. Department of Agriculture	2353057000
U.S. Department of Agriculture
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative
Center for Arthropod Management Technologies	IIP‐1821936
Center for Arthropod Management Technologies

Keywords

RNA interference
Tribolium castaneum
iap
red flour beetle
survivin

ASJC Scopus subject areas

Physiology
Biochemistry
Insect Science

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10.1002/arch.21689

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title = "Evaluation of inhibitor of apoptosis genes as targets for RNAi-mediated control of insect pests",

abstract = "Apoptosis has been widely studied from mammals to insects. Inhibitor of apoptosis (IAP) protein is a negative regulator of apoptosis. Recent studies suggest that iap genes could be excellent targets for RNA interference (RNAi)-mediated control of insect pests. However, not much is known about iap genes in one of the well-known insect model species, Tribolium castaneum. The orthologues of five iap genes were identified in T. castaneum by searching its genome at NCBI (https://www.ncbi.nlm.nih.gov/) and UniProt (https://www.uniprot.org/) databases using Drosophila melanogaster and Aedes aegypti IAP protein sequences as queries. RNAi assays were performed in T. castaneum cell line (TcA) and larvae. The knockdown of iap1 gene induced a distinct apoptotic phenotype in TcA cells and induced 91\% mortality in T. castaneum larvae. Whereas, knockdown of iap5 resulted in a decrease in cell proliferation in TcA cells and developmental defects in T. castaneum larvae which led to 100\% mortality. Knockdown of the other three iap genes identified did not cause a significant effect on cells or insects. These data increase our understanding of iap genes in insects and provide opportunities for developing iap1 and iap5 as targets for RNAi-based insect pest control.",

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doi = "10.1002/arch.21689",

language = "English",

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Evaluation of inhibitor of apoptosis genes as targets for RNAi-mediated control of insect pests

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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