Evaluation of microbiome in primary and permanent dentition in grade C periodontitis in young individuals

Sungeun Stephanie Koo, Jussara G. Fernandes, Lu Li, Hong Huang, Ikramuddin Aukhil, Peter Harrison, Patricia I. Diaz, Luciana M. Shaddox

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background: The aim of the present study was to evaluate the subgingival microbiome in patients with grade C molar-incisor pattern periodontitis (C-MIP) affecting the primary or permanent dentitions. Methods: DNA was isolated from subgingival biofilm samples from diseased and healthy sites from 45 C-MIP patients and subjected to phylogenetic microarray analysis. C-MIP sites were compared between children affected in the primary to those affected in the permanent dentitions. Within-subject differences between C-MIP-affected sites and dentition-matched healthy sites were also evaluated. Results: C-MIP sites of subjects affected in the primary dentition showed partially overlapping but distinct microbial communities from C-MIP permanent dentition sites (p < 0.05). Differences were due to increased levels in primary C-MIP sites of certain species of the genera Capnocytophaga and Leptotrichia, while C-MIP permanent dentition sites showed higher prevalence of Filifactor alocis. Aggregatibacter actinomycetemcomitans (Aa) was among species seen in high prevalence and levels in both primary and permanent C-MIP sites. Moreover, both permanent and primary C-MIP sites showed distinct microbial communities when compared to dentition-matched healthy sites in the same subject (p < 0.01). Conclusions: Primary and permanent teeth with C-MIP showed a dysbiotic microbiome, with children affected in the primary dentition showing a distinct profile from those affected in the permanent dentition. However, Aa was enriched in both primary and permanent diseased sites, confirming that this microorganism is implicated in C-MIP in both dentitions.

Original languageEnglish
JournalJournal of Periodontology
DOIs
StateAccepted/In press - 2024

Bibliographical note

Publisher Copyright:
© 2024 American Academy of Periodontology.

Funding

The authors would like to thank the doctors and staff at the Leon (Drs. John Bidwell, Edward Zapert, and Dan Cober), Duval County Health Departments (Drs. Phillis Varnado and Renata Sturtz), and the University of Florida (UF) dental clinics and surrounding referring clinics for their assistance in coordinating our visits to their clinics, patient referrals, and dental care. This study was supported by the National Institute of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCRR01DE019456).

FundersFunder number
National Institutes of Health (NIH)
National Institute of Dental and Craniofacial ResearchNIH/NIDCRR01DE019456

    Keywords

    • aggressive periodontitis
    • children
    • microbiome
    • primary dentition

    ASJC Scopus subject areas

    • Periodontics

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