Evaluation of Novel 64Cu-Labeled Theranostic Gadolinium-Based Nanoprobes in HepG2 Tumor-Bearing Nude Mice

Pengcheng Hu, Dengfeng Cheng, Tao Huang, Anna B. Banizs, Jie Xiao, Guobing Liu, Quan Chen, Yuenan Wang, Jiang He, Hongcheng Shi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Radiation therapy of liver cancer is limited by low tolerance of the liver to radiation. Radiosensitizers can effectively reduce the required radiation dose. AGuIX nanoparticles are small, multifunctional gadolinium-based nanoparticles that can carry radioisotopes or fluorescent markers for single-photon emission computed tomography (SPECT), positron emission tomography (PET), fluorescence imaging, and even multimodality imaging. In addition, due to the high atomic number of gadolinium, it can also serve as a tumor radiation sensitizer. It is critical to define the biodistribution and pharmacokinetics of these gadolinium-based nanoparticles to quantitate the magnitude and duration of their retention within the tumor microenvironment during radiotherapy. Therefore, in this study, we successfully labeled AGuIX with 64Cu through the convenient built-in chelator. The biodistribution studies indicated that the radiotracer 64Cu-AGuIX accumulates to high levels in the HepG2 xenograft of nude mice, suggesting that it would be a potential theranostic nanoprobe for image-guided radiotherapy in HCC. We also used a transmission electron microscope to confirm AGuIX uptake in the HepG2 cells. In radiation therapy studies, a decrease in 18F-FDG uptake was observed in the xenografts of the nude mice irradiated with AGuIX, which was injected 1 h before. These results provide proof-of-concept that AGuIX can be used as a theranostic radiosensitizer for PET imaging to guide radiotherapy for liver cancer.

Original languageEnglish
Article number523
JournalNanoscale Research Letters
Volume12
DOIs
StatePublished - 2017

Bibliographical note

Funding Information:
Research reported in this publication was partially supported by the National Cancer Institute (CCSG P30 CA44579). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Publisher Copyright:
© 2017, The Author(s).

Keywords

  • Cancer
  • MRI
  • Molecular imaging
  • Nanoparticles
  • PET
  • Radiotherapy

ASJC Scopus subject areas

  • Materials Science (all)
  • Condensed Matter Physics

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