Currently, traditional outdoor and raceway aquaculture systems in the United States produce less than 1% of the world's shrimp. With 90% of domestic demands being imported from abroad, recirculating aquaculture systems (RAS) have been developed to produce shrimp at high densities in tanks located in a controlled environment. The sustainability of RAS shrimp production is critical to the development of this industry in the U.S. By placing the RAS tanks in a controlled environment, e.g., greenhouse or warehouse, year round production can be realized. Energy costs are a vital concern, since the temperature of the water in the tanks needs to be maintained at an elevated temperature (i.e. 30°C). One of the primary energy losses from the system is due to evaporation from the tanks. This phenomenon constitutes more than 50% of the total energy losses from the system although little data is available. Water conservation is also an important consideration, because the water needs to be replaced. In order to assess the energy losses due to evaporation in indoor production system for saltwater shrimp, the research experiment was conducted in an environmental chamber in the Biosystems and Agricultural Department at the University of Kentucky. The interior environmental conditions i.e., air temperature and relative humidity were controlled. Energy and water losses due to evaporation were measured along with investigating the influence of aeration on evaporation.