Evidence for a Competitive-Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA

Kevin D. Sarge, E. Stuart Maxwell

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We have previously shown that a 5′-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA 18 S rRNA and mRNA.

Original languageEnglish
Pages (from-to)234-238
Number of pages5
JournalFEBS Letters
Volume294
Issue number3
DOIs
StatePublished - Dec 9 1991

Bibliographical note

Funding Information:
.4ckno.&,deemcnts- Initial aspects of tints work wcrc supported by PHS Grant L'A38015 from NCI and BRSG Grant RR7071 {E.S.M.).

Keywords

  • Intermolecular RNA:RNA hybridization
  • Ribosome
  • Translation
  • mRNA
  • rRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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