Abstract
We have previously shown that a 5′-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA 18 S rRNA and mRNA.
Original language | English |
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Pages (from-to) | 234-238 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 294 |
Issue number | 3 |
DOIs | |
State | Published - Dec 9 1991 |
Bibliographical note
Funding Information:.4ckno.&,deemcnts- Initial aspects of tints work wcrc supported by PHS Grant L'A38015 from NCI and BRSG Grant RR7071 {E.S.M.).
Keywords
- Intermolecular RNA:RNA hybridization
- Ribosome
- Translation
- mRNA
- rRNA
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology