Evidence for a Competitive-Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA

Kevin D. Sarge; E. Stuart Maxwell

doi:10.1016/0014-5793(91)81437-D

Evidence for a Competitive-Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA

Kevin D. Sarge
, E. Stuart Maxwell

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We have previously shown that a 5′-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA 18 S rRNA and mRNA.

Original language	English
Pages (from-to)	234-238
Number of pages	5
Journal	FEBS Letters
Volume	294
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(91)81437-D
State	Published - Dec 9 1991

Bibliographical note

Funding Information:
.4ckno.&,deemcnts- Initial aspects of tints work wcrc supported by PHS Grant L'A38015 from NCI and BRSG Grant RR7071 {E.S.M.).

Funding

.4ckno.&,deemcnts- Initial aspects of tints work wcrc supported by PHS Grant L'A38015 from NCI and BRSG Grant RR7071 {E.S.M.).

Funders	Funder number
BRSG	RR7071
PHS	L'A38015
National Childhood Cancer Registry – National Cancer Institute	R23CA038015

Keywords

Intermolecular RNA:RNA hybridization
Ribosome
Translation
mRNA
rRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)81437-D

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title = "Evidence for a Competitive-Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA",

abstract = "We have previously shown that a 5′-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA 18 S rRNA and mRNA.",

keywords = "Intermolecular RNA:RNA hybridization, Ribosome, Translation, mRNA, rRNA",

author = "Sarge, \{Kevin D.\} and Maxwell, \{E. Stuart\}",

note = "Funding Information: .4ckno.\&,deemcnts- Initial aspects of tints work wcrc supported by PHS Grant L'A38015 from NCI and BRSG Grant RR7071 \{E.S.M.).",

year = "1991",

month = dec,

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doi = "10.1016/0014-5793(91)81437-D",

language = "English",

volume = "294",

pages = "234--238",

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T1 - Evidence for a Competitive-Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA

AU - Sarge, Kevin D.

AU - Maxwell, E. Stuart

N1 - Funding Information: .4ckno.&,deemcnts- Initial aspects of tints work wcrc supported by PHS Grant L'A38015 from NCI and BRSG Grant RR7071 {E.S.M.).

PY - 1991/12/9

Y1 - 1991/12/9

N2 - We have previously shown that a 5′-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA 18 S rRNA and mRNA.

AB - We have previously shown that a 5′-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA 18 S rRNA and mRNA.

KW - Intermolecular RNA:RNA hybridization

KW - Ribosome

KW - Translation

KW - mRNA

KW - rRNA

UR - https://www.scopus.com/pages/publications/0025931608

UR - https://www.scopus.com/pages/publications/0025931608#tab=citedBy

U2 - 10.1016/0014-5793(91)81437-D

DO - 10.1016/0014-5793(91)81437-D

M3 - Article

C2 - 1756865

AN - SCOPUS:0025931608

SN - 0014-5793

VL - 294

SP - 234

EP - 238

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Evidence for a Competitive-Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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