Evidence for superoxide radical production in peroxynitrite decomposition

L. Y. Zang; X. Shi

Evidence for superoxide radical production in peroxynitrite decomposition

L. Y. Zang, X. Shi

Toxicology and Cancer Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Peroxynitrite decomposition was investigated by ESR spin trapping. The spin trap used was 5,5-dimethyl-1-pyrroline N-oxide (DMPO). A mixture of peroxynitrite and DMPO generated predominantly DMPO-O₂^- adduct. A combination of SOD and catalase suppressed the formation of DMPO-O₂^-. The DMPO-O₂^- signal reached its maximum at pH lower than 7 and decreased as pH increased. The DMPO-O₂^- signal also depended on peroxynitrite concentration with maximum signal intensity appearing at 4.2 mM. The results demonstrate that peroxynitrite decomposition generates O₂^-. Since reaction of H₂O₂ with NO₂^- generates peroxynitrite, the results point out a pathway for conversion of H₂O₂ to O₂^- via peroxynitrite as an intermediate.

Original language	English
Pages (from-to)	355-360
Number of pages	6
Journal	Biochemistry and Molecular Biology International
Volume	37
Issue number	2
State	Published - 1995

Keywords

ESR spin trapping
O generation
Peroxynitrite decomposition

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

Cite this

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title = "Evidence for superoxide radical production in peroxynitrite decomposition",

abstract = "Peroxynitrite decomposition was investigated by ESR spin trapping. The spin trap used was 5,5-dimethyl-1-pyrroline N-oxide (DMPO). A mixture of peroxynitrite and DMPO generated predominantly DMPO-O2- adduct. A combination of SOD and catalase suppressed the formation of DMPO-O2-. The DMPO-O2- signal reached its maximum at pH lower than 7 and decreased as pH increased. The DMPO-O2- signal also depended on peroxynitrite concentration with maximum signal intensity appearing at 4.2 mM. The results demonstrate that peroxynitrite decomposition generates O2-. Since reaction of H2O2 with NO2- generates peroxynitrite, the results point out a pathway for conversion of H2O2 to O2- via peroxynitrite as an intermediate.",

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author = "Zang, {L. Y.} and X. Shi",

year = "1995",

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TY - JOUR

T1 - Evidence for superoxide radical production in peroxynitrite decomposition

AU - Zang, L. Y.

AU - Shi, X.

PY - 1995

Y1 - 1995

N2 - Peroxynitrite decomposition was investigated by ESR spin trapping. The spin trap used was 5,5-dimethyl-1-pyrroline N-oxide (DMPO). A mixture of peroxynitrite and DMPO generated predominantly DMPO-O2- adduct. A combination of SOD and catalase suppressed the formation of DMPO-O2-. The DMPO-O2- signal reached its maximum at pH lower than 7 and decreased as pH increased. The DMPO-O2- signal also depended on peroxynitrite concentration with maximum signal intensity appearing at 4.2 mM. The results demonstrate that peroxynitrite decomposition generates O2-. Since reaction of H2O2 with NO2- generates peroxynitrite, the results point out a pathway for conversion of H2O2 to O2- via peroxynitrite as an intermediate.

AB - Peroxynitrite decomposition was investigated by ESR spin trapping. The spin trap used was 5,5-dimethyl-1-pyrroline N-oxide (DMPO). A mixture of peroxynitrite and DMPO generated predominantly DMPO-O2- adduct. A combination of SOD and catalase suppressed the formation of DMPO-O2-. The DMPO-O2- signal reached its maximum at pH lower than 7 and decreased as pH increased. The DMPO-O2- signal also depended on peroxynitrite concentration with maximum signal intensity appearing at 4.2 mM. The results demonstrate that peroxynitrite decomposition generates O2-. Since reaction of H2O2 with NO2- generates peroxynitrite, the results point out a pathway for conversion of H2O2 to O2- via peroxynitrite as an intermediate.

KW - ESR spin trapping

KW - O generation

KW - Peroxynitrite decomposition

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JO - Biochemistry and Molecular Biology International

JF - Biochemistry and Molecular Biology International

IS - 2

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Evidence for superoxide radical production in peroxynitrite decomposition

Abstract

Keywords

ASJC Scopus subject areas

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