Evidence for the inhibition of fertilization in vitro by anti-ZP3 antisera derived from DNA vaccine

Meng Fei Yu; Wen Ning Fang; Gao Feng Xiong; Ying Yang; Jing Pian Peng

doi:10.1016/j.vaccine.2011.04.130

Evidence for the inhibition of fertilization in vitro by anti-ZP3 antisera derived from DNA vaccine

Meng Fei Yu
, Wen Ning Fang
, Gao Feng Xiong
, Ying Yang
, Jing Pian Peng

Pharmacology and Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Previously we have found that DNA vaccine, pCMV4-rZPC' can generate specific antibodies against rabbit ZPC (amino acid 263-415, rZPC'), which binds to ovarian ZP and leads to a significant reduction of fertility in vivo. The purpose of this study was to evaluate the effect of antisera from pCMV4-rZPC^'-immunized mice on sperm-oocyte interaction in vitro. The effect of antisera from DNA vaccine-immunized mice on fertilization and early embryonic development was studied using an in vitro fertilization system. The results showed that the antisera supplemented in fertilization medium (10%, v/v) significantly decreased the rate of fertilization compared to that of control groups (P<0.05); whereas the antisera showed no significant effect on the rate of fertilization when ZP-free eggs were used. Moreover, the antisera pre-neutralized with mouse soluble zona pellucida lost the capacity to inhibit fertilization when compared with that of control groups. In addition, the antisera showed no detrimental effect on early developmental potential of mouse embryos in vitro. Taken together, our study provided herein direct evidence showing that antisera generated by DNA vaccine can block sperm-egg recognition during fertilization via targeting the oocyte ZP proteins.

Original language	English
Pages (from-to)	4933-4939
Number of pages	7
Journal	Vaccine
Volume	29
Issue number	31
DOIs	https://doi.org/10.1016/j.vaccine.2011.04.130
State	Published - Jul 12 2011

Bibliographical note

Funding Information:
The authors appreciate Dr. Haibin Wang (Institute of Zoology, Chinese Academy of Sciences, Beijing, China) for his careful reading of this manuscript. This work was supported by grants from the National Basic Research Program of China [No. 2011CB944402] and the K nowledge Innovation Program of Chinese Academy of Sciences [KSCX2-EW-R-06].

Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.

Funding

The authors appreciate Dr. Haibin Wang (Institute of Zoology, Chinese Academy of Sciences, Beijing, China) for his careful reading of this manuscript. This work was supported by grants from the National Basic Research Program of China [No. 2011CB944402] and the K nowledge Innovation Program of Chinese Academy of Sciences [KSCX2-EW-R-06].

Funders	Funder number
Chinese Academy of Sciences	KSCX2-EW-R-06
National Key Research and Development Program of China	2011CB944402

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Anti-fertility
Antiserum
DNA vaccine
In vitro fertilization

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

Access to Document

10.1016/j.vaccine.2011.04.130

Cite this

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title = "Evidence for the inhibition of fertilization in vitro by anti-ZP3 antisera derived from DNA vaccine",

abstract = "Previously we have found that DNA vaccine, pCMV4-rZPC' can generate specific antibodies against rabbit ZPC (amino acid 263-415, rZPC'), which binds to ovarian ZP and leads to a significant reduction of fertility in vivo. The purpose of this study was to evaluate the effect of antisera from pCMV4-rZPC'-immunized mice on sperm-oocyte interaction in vitro. The effect of antisera from DNA vaccine-immunized mice on fertilization and early embryonic development was studied using an in vitro fertilization system. The results showed that the antisera supplemented in fertilization medium (10\%, v/v) significantly decreased the rate of fertilization compared to that of control groups (P<0.05); whereas the antisera showed no significant effect on the rate of fertilization when ZP-free eggs were used. Moreover, the antisera pre-neutralized with mouse soluble zona pellucida lost the capacity to inhibit fertilization when compared with that of control groups. In addition, the antisera showed no detrimental effect on early developmental potential of mouse embryos in vitro. Taken together, our study provided herein direct evidence showing that antisera generated by DNA vaccine can block sperm-egg recognition during fertilization via targeting the oocyte ZP proteins.",

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author = "Yu, \{Meng Fei\} and Fang, \{Wen Ning\} and Xiong, \{Gao Feng\} and Ying Yang and Peng, \{Jing Pian\}",

note = "Funding Information: The authors appreciate Dr. Haibin Wang (Institute of Zoology, Chinese Academy of Sciences, Beijing, China) for his careful reading of this manuscript. This work was supported by grants from the National Basic Research Program of China [No. 2011CB944402] and the K nowledge Innovation Program of Chinese Academy of Sciences [KSCX2-EW-R-06]. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

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AU - Peng, Jing Pian

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AB - Previously we have found that DNA vaccine, pCMV4-rZPC' can generate specific antibodies against rabbit ZPC (amino acid 263-415, rZPC'), which binds to ovarian ZP and leads to a significant reduction of fertility in vivo. The purpose of this study was to evaluate the effect of antisera from pCMV4-rZPC'-immunized mice on sperm-oocyte interaction in vitro. The effect of antisera from DNA vaccine-immunized mice on fertilization and early embryonic development was studied using an in vitro fertilization system. The results showed that the antisera supplemented in fertilization medium (10%, v/v) significantly decreased the rate of fertilization compared to that of control groups (P<0.05); whereas the antisera showed no significant effect on the rate of fertilization when ZP-free eggs were used. Moreover, the antisera pre-neutralized with mouse soluble zona pellucida lost the capacity to inhibit fertilization when compared with that of control groups. In addition, the antisera showed no detrimental effect on early developmental potential of mouse embryos in vitro. Taken together, our study provided herein direct evidence showing that antisera generated by DNA vaccine can block sperm-egg recognition during fertilization via targeting the oocyte ZP proteins.

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Evidence for the inhibition of fertilization in vitro by anti-ZP3 antisera derived from DNA vaccine

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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