Evidence of gene introgression in apple using RAPD markers

Richard E. Durham, Schuyler S. Korban

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


A genomic remnant of Malus floribunda clone 821 introgressed into the cultivated apple M. x domestica Borkh. was identified using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). Using a set of 59 oligonucleotide decamer primers, polymorphic DNA markers were identified among three pooled DNA samples. Based on the presence or absence of bands among bulked apple scab-resistant selections and cultivars, bulked scab-susceptible cultivars, and a M. floribunda clone 821 sample, one primer, A 15, identified amplified fragments in the scab-resistant bulked sample that was also unique to the M. floribunda clone 821. The unique band from M. floribunda clone 821 was amplified in four out of 17 scab-resistant selections/cultivars. This RAPD, designated OA15900, identifies an introgressed fragment that has as yet no known function.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
Issue number1-2
StatePublished - Jan 1994


  • DNA markers
  • Malus
  • PCR
  • RAPDs
  • Venturia inaequalis
  • apple
  • apple scab
  • gene introgression
  • resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture


Dive into the research topics of 'Evidence of gene introgression in apple using RAPD markers'. Together they form a unique fingerprint.

Cite this