Evidence of gene introgression in apple using RAPD markers

Richard E. Durham; Schuyler S. Korban

doi:10.1007/BF00023582

Evidence of gene introgression in apple using RAPD markers

Richard E. Durham
, Schuyler S. Korban

Horticulture

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A genomic remnant of Malus floribunda clone 821 introgressed into the cultivated apple M. x domestica Borkh. was identified using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). Using a set of 59 oligonucleotide decamer primers, polymorphic DNA markers were identified among three pooled DNA samples. Based on the presence or absence of bands among bulked apple scab-resistant selections and cultivars, bulked scab-susceptible cultivars, and a M. floribunda clone 821 sample, one primer, A 15, identified amplified fragments in the scab-resistant bulked sample that was also unique to the M. floribunda clone 821. The unique band from M. floribunda clone 821 was amplified in four out of 17 scab-resistant selections/cultivars. This RAPD, designated OA15₉₀₀, identifies an introgressed fragment that has as yet no known function.

Original language	English
Pages (from-to)	109-114
Number of pages	6
Journal	Euphytica
Volume	79
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00023582
State	Published - Jan 1994

Keywords

DNA markers
Malus
PCR
RAPDs
Venturia inaequalis
apple
apple scab
gene introgression
resistance

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science
Horticulture

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10.1007/BF00023582

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@article{18f6eb4af2144fb8b9514beba8651d93,

title = "Evidence of gene introgression in apple using RAPD markers",

abstract = "A genomic remnant of Malus floribunda clone 821 introgressed into the cultivated apple M. x domestica Borkh. was identified using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). Using a set of 59 oligonucleotide decamer primers, polymorphic DNA markers were identified among three pooled DNA samples. Based on the presence or absence of bands among bulked apple scab-resistant selections and cultivars, bulked scab-susceptible cultivars, and a M. floribunda clone 821 sample, one primer, A 15, identified amplified fragments in the scab-resistant bulked sample that was also unique to the M. floribunda clone 821. The unique band from M. floribunda clone 821 was amplified in four out of 17 scab-resistant selections/cultivars. This RAPD, designated OA15900, identifies an introgressed fragment that has as yet no known function.",

keywords = "DNA markers, Malus, PCR, RAPDs, Venturia inaequalis, apple, apple scab, gene introgression, resistance",

author = "Durham, \{Richard E.\} and Korban, \{Schuyler S.\}",

year = "1994",

month = jan,

doi = "10.1007/BF00023582",

language = "English",

volume = "79",

pages = "109--114",

number = "1-2",

}

TY - JOUR

T1 - Evidence of gene introgression in apple using RAPD markers

AU - Durham, Richard E.

AU - Korban, Schuyler S.

PY - 1994/1

Y1 - 1994/1

N2 - A genomic remnant of Malus floribunda clone 821 introgressed into the cultivated apple M. x domestica Borkh. was identified using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). Using a set of 59 oligonucleotide decamer primers, polymorphic DNA markers were identified among three pooled DNA samples. Based on the presence or absence of bands among bulked apple scab-resistant selections and cultivars, bulked scab-susceptible cultivars, and a M. floribunda clone 821 sample, one primer, A 15, identified amplified fragments in the scab-resistant bulked sample that was also unique to the M. floribunda clone 821. The unique band from M. floribunda clone 821 was amplified in four out of 17 scab-resistant selections/cultivars. This RAPD, designated OA15900, identifies an introgressed fragment that has as yet no known function.

AB - A genomic remnant of Malus floribunda clone 821 introgressed into the cultivated apple M. x domestica Borkh. was identified using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). Using a set of 59 oligonucleotide decamer primers, polymorphic DNA markers were identified among three pooled DNA samples. Based on the presence or absence of bands among bulked apple scab-resistant selections and cultivars, bulked scab-susceptible cultivars, and a M. floribunda clone 821 sample, one primer, A 15, identified amplified fragments in the scab-resistant bulked sample that was also unique to the M. floribunda clone 821. The unique band from M. floribunda clone 821 was amplified in four out of 17 scab-resistant selections/cultivars. This RAPD, designated OA15900, identifies an introgressed fragment that has as yet no known function.

KW - DNA markers

KW - Malus

KW - PCR

KW - RAPDs

KW - Venturia inaequalis

KW - apple

KW - apple scab

KW - gene introgression

KW - resistance

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JO - Euphytica

JF - Euphytica

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ER -

Evidence of gene introgression in apple using RAPD markers

Abstract

Keywords

ASJC Scopus subject areas

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