Evolution of the vertebrate claudin gene family: Insights from a basal vertebrate, the sea lamprey

Christian Mukendi, Nicholas Dean, Rushil Lala, Jeramiah Smith, Marianne E. Bronner, Natalya V. Nikitina

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Claudins are major constituents of tight junctions, contributing both to their intercellular sealing and selective permeability properties. While claudins and claudin-like molecules are present in some invertebrates, the association of claudins with tight junctions has been conclusively documented only in vertebrates. Here we report the sequencing, phylogenetic analysis and comprehensive spatiotemporal expression analysis of the entire claudin gene family in the basal extant vertebrate, the sea lamprey. Our results demonstrate that clear orthologues to about half of all mammalian claudins are present in the lamprey, suggesting that at least one round of whole genome duplication contributed to the diversification of this gene family. Expression analysis revealed that claudins are expressed in discrete and specific domains, many of which represent vertebrate-specific innovations, such as in cranial ectodermal placodes and the neural crest; whereas others represent structures characteristic of chordates, e.g. pronephros, notochord, somites, endostyle and pharyngeal arches. By comparing the embryonic expression of claudins in the lamprey to that of other vertebrates, we found that ancestral expression patterns were often preserved in higher vertebrates. Morpholino mediated loss of Cldn3b demonstrated a functional role for this protein in placode and pharyngeal arch morphogenesis. Taken together, our data provide novel insights into the origins and evolution of the claudin gene family and the significance of claudin proteins in the evolution of vertebrates.

Original languageEnglish
Pages (from-to)39-51
Number of pages13
JournalInternational Journal of Developmental Biology
Volume60
Issue number1-3
DOIs
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 UPV/EHU Press.

Keywords

  • Claudin
  • Embryo
  • Lamprey
  • Vertebrate primordia

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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