Ex vivo study of telluride nanowires in minigut

Yijun Qi; Enzheng Shi; Nathan Peroutka-Bigus; Bryan Bellaire; Michael Wannemuehler; Albert Jergens; Terrence Barrett; Yue Wu; Qun Wang

doi:10.1166/jbn.2018.2578

Ex vivo study of telluride nanowires in minigut

Yijun Qi, Enzheng Shi, Nathan Peroutka-Bigus, Bryan Bellaire, Michael Wannemuehler, Albert Jergens, Terrence Barrett, Yue Wu, Qun Wang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Compound semiconductor nanomaterials, such as telluride nanowires, nanorods, and nanoparticles, have many unique properties for wide range of potential applications. The interaction between organoids (a biological system) and telluride nanowires is a crucial research area for human health in terms of its safety concerns. In this study, we demonstrated a case study on Bi ₂ Te ₃ nanowires. Through live/dead cell viability testing, bright-light image analysis, and surface area calculations, we showed that 50μg/mL Bi ₂ Te ₃ exerts minimum influence on shrinking crypts and disrupting lumen structure, which causes unhealthy growth. Within this optimal concentration, Bi ₂ Te ₃ nanowires can stay as a stable and non-toxic material inside the intestine. Unlike the previous studies of the cytotoxicity of Telluride nanomaterials interacting with single type of cells, our research demonstrated the first study of the interactions of engineered Telluride nanomaterials with a real complex gastrointestinal tract system as our primary small intestinal crypts were directly isolated from mice and grew into a self-renewable system with various types of cells and different cell pathways, which has the capability to mimic a fully functional intestinal epithelium layer for a realistic study inside the gastrointestinal tract. Most importantly, we showed that Bi ₂ Te ₃ nanowires, under infrared exposure, can act as a potential shield to stimulate cell viability and improve cell survivability.

Original language	English
Pages (from-to)	978-986
Number of pages	9
Journal	Journal of Biomedical Nanotechnology
Volume	14
Issue number	5
DOIs	https://doi.org/10.1166/jbn.2018.2578
State	Published - May 2018

Bibliographical note

Publisher Copyright:
Copyright © 2018 American Scientific Publishers All rights reserved.

Funding

vation and further impact on the cells at molecular level are still under investigation, which could be related with Acknowledgments: We thank Tracey Pepper Stewart at the electrical, optical, and therImPa: l20p3ro.5p6er.t2ie4s1.o1f28t hOesne: ThuI,o w24a SMtaatye 2U0n1iv8e 1rs1it:y15B:e2s4sey Microscopy Facility for pro-nanowires. Although the mechanism hCopyright: American Scientific Publishersas not been studied viding equipmentand technologiesfor cell histology imag- yet, sev31eral4243assumptions can be made based oDelivered by Ingentan other ing.WethankCrohn’sandColitisFoundationofAmerica studies. Itissuggestedthatthemechanismofnan- (CCFA) Career Award (No. 348137), PhRMA Foundation otoxicity is related with the generation of reactive oxygen Research Starter Award (No. RSGTMT17), and McGee- species (ROS).42 IR exposure first caused cell inflamma-Wagner Interdisciplinary Research Foundation supported tion, which leads to ROS formations, further caused cell this research. The research was also supported (TAB) by death through free-radical mechanisms. However, telluride NIH (2RO1 DK095662) and VA Merit (1I01CX001353) nanowires are different compared with silicon nanowires. grants. They are thinner, smaller, and have well thermoelectrical and photothermal properties.43 With its thermoelectrical properties, a proper concentration is able to protect our biological system away from IR exposure. The unique shielding effect of nanowires could provide a potential solution for protecting stem cells under radiation or other medical treatment. We thank Tracey Pepper Stewart at Iowa State University Bessey Microscopy Facility for providing equipment and technologies for cell histology imaging. We thank Crohn's and Colitis Foundation of America (CCFA) Career Award (No. 348137), PhRMA Foundation Research Starter Award (No. RSGTMT17), and McGee- Wagner Interdisciplinary Research Foundation supported this research. The research was also supported (TAB) by NIH (2RO1 DK095662) and VA Merit (1I01CX001353) grants.

Funders	Funder number
Interdisciplinary Research Foundation
PhRMA Foundation Research Starter
Tracey Pepper Stewart at Iowa State University Bessey Microscopy Facility
VA Merit Award	1I01CX001353
National Institutes of Health (NIH)	2RO1 DK095662
National Institutes of Health (NIH)
Crohn's and Colitis Foundation of America	348137
Crohn's and Colitis Foundation of America
Pharmaceutical Research and Manufacturers of America Foundation	RSGTMT17
Pharmaceutical Research and Manufacturers of America Foundation

Keywords

Bismuth telluride
Minigut
Nanowires
Organoids
Toxicity

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1166/jbn.2018.2578

Cite this

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title = "Ex vivo study of telluride nanowires in minigut",

abstract = " Compound semiconductor nanomaterials, such as telluride nanowires, nanorods, and nanoparticles, have many unique properties for wide range of potential applications. The interaction between organoids (a biological system) and telluride nanowires is a crucial research area for human health in terms of its safety concerns. In this study, we demonstrated a case study on Bi 2 Te 3 nanowires. Through live/dead cell viability testing, bright-light image analysis, and surface area calculations, we showed that 50μg/mL Bi 2 Te 3 exerts minimum influence on shrinking crypts and disrupting lumen structure, which causes unhealthy growth. Within this optimal concentration, Bi 2 Te 3 nanowires can stay as a stable and non-toxic material inside the intestine. Unlike the previous studies of the cytotoxicity of Telluride nanomaterials interacting with single type of cells, our research demonstrated the first study of the interactions of engineered Telluride nanomaterials with a real complex gastrointestinal tract system as our primary small intestinal crypts were directly isolated from mice and grew into a self-renewable system with various types of cells and different cell pathways, which has the capability to mimic a fully functional intestinal epithelium layer for a realistic study inside the gastrointestinal tract. Most importantly, we showed that Bi 2 Te 3 nanowires, under infrared exposure, can act as a potential shield to stimulate cell viability and improve cell survivability.",

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AU - Shi, Enzheng

AU - Peroutka-Bigus, Nathan

AU - Bellaire, Bryan

AU - Wannemuehler, Michael

AU - Jergens, Albert

AU - Barrett, Terrence

AU - Wu, Yue

AU - Wang, Qun

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N2 - Compound semiconductor nanomaterials, such as telluride nanowires, nanorods, and nanoparticles, have many unique properties for wide range of potential applications. The interaction between organoids (a biological system) and telluride nanowires is a crucial research area for human health in terms of its safety concerns. In this study, we demonstrated a case study on Bi 2 Te 3 nanowires. Through live/dead cell viability testing, bright-light image analysis, and surface area calculations, we showed that 50μg/mL Bi 2 Te 3 exerts minimum influence on shrinking crypts and disrupting lumen structure, which causes unhealthy growth. Within this optimal concentration, Bi 2 Te 3 nanowires can stay as a stable and non-toxic material inside the intestine. Unlike the previous studies of the cytotoxicity of Telluride nanomaterials interacting with single type of cells, our research demonstrated the first study of the interactions of engineered Telluride nanomaterials with a real complex gastrointestinal tract system as our primary small intestinal crypts were directly isolated from mice and grew into a self-renewable system with various types of cells and different cell pathways, which has the capability to mimic a fully functional intestinal epithelium layer for a realistic study inside the gastrointestinal tract. Most importantly, we showed that Bi 2 Te 3 nanowires, under infrared exposure, can act as a potential shield to stimulate cell viability and improve cell survivability.

AB - Compound semiconductor nanomaterials, such as telluride nanowires, nanorods, and nanoparticles, have many unique properties for wide range of potential applications. The interaction between organoids (a biological system) and telluride nanowires is a crucial research area for human health in terms of its safety concerns. In this study, we demonstrated a case study on Bi 2 Te 3 nanowires. Through live/dead cell viability testing, bright-light image analysis, and surface area calculations, we showed that 50μg/mL Bi 2 Te 3 exerts minimum influence on shrinking crypts and disrupting lumen structure, which causes unhealthy growth. Within this optimal concentration, Bi 2 Te 3 nanowires can stay as a stable and non-toxic material inside the intestine. Unlike the previous studies of the cytotoxicity of Telluride nanomaterials interacting with single type of cells, our research demonstrated the first study of the interactions of engineered Telluride nanomaterials with a real complex gastrointestinal tract system as our primary small intestinal crypts were directly isolated from mice and grew into a self-renewable system with various types of cells and different cell pathways, which has the capability to mimic a fully functional intestinal epithelium layer for a realistic study inside the gastrointestinal tract. Most importantly, we showed that Bi 2 Te 3 nanowires, under infrared exposure, can act as a potential shield to stimulate cell viability and improve cell survivability.

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KW - Organoids

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Ex vivo study of telluride nanowires in minigut

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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