Examining lethality risk for rodent studies of primary blast lung injury

William Brad Hubbard, Christina Hall, Venkata Siva Sai Sujith Sajja, Erin Lavik, Pamela Vande Vord

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

11 Scopus citations

Abstract

While protective measures have been taken to mitigate injury to the thorax during a blast exposure, primary blast lung injury (PBLI) is still evident in mounted/in vehicle cases during military conflicts. Moreover, civilians, who are unprotected from blast exposure, can be severely harmed by terrorist attacks that use improvised explosive devices (IEDs). Since the lungs are the most susceptible organ due to their air-filled nature, PBLI is one of the most serious injuries seen in civilian blast cases. Determining lethality threshold for rodent studies is crucial to guide experimental designs centered on therapies for survival after PBLI or mechanistic understanding of the injury itself. Using an Advanced Blast Simulator, unprotected rats were exposed to a whole body blast to induce PBLI. The one-hour survival rate was assessed to determine operating conditions for a 50% lethality rate. Macroscopic and histological analysis of lung was conducted using hematoxylin and eosin staining. Results demonstrated lethality risk trends based on static blast overpressure (BOP) for rodent models, which may help standardized animal studies and contribute to scaling to the human level. The need for a standardized method of producing PBLI is pressing and establishing standard curves, such as a lethality risk curve for lung blasts, is crucial for this condensing of BOP methods.

Original languageEnglish
Title of host publication51st Annual Rocky Mountain Bioengineering Symposium, RMBS 2014 and 51st International ISA Biomedical Sciences Instrumentation Symposium 2014
PublisherISA - Instrumentation, Systems, and Automation Society
Pages92-99
Number of pages8
ISBN (Print)9781632663917
StatePublished - 2014
Event51st Annual Rocky Mountain Bioengineering Symposium, RMBS 2014 and 51st International ISA Biomedical Sciences Instrumentation Symposium 2014 - Denver, CO, United States
Duration: Apr 4 2014Apr 6 2014

Publication series

Name51st Annual Rocky Mountain Bioengineering Symposium, RMBS 2014 and 51st International ISA Biomedical Sciences Instrumentation Symposium 2014

Conference

Conference51st Annual Rocky Mountain Bioengineering Symposium, RMBS 2014 and 51st International ISA Biomedical Sciences Instrumentation Symposium 2014
Country/TerritoryUnited States
CityDenver, CO
Period4/4/144/6/14

Keywords

  • Blast
  • Lethality risk
  • Lung injury
  • Rats

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Instrumentation

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