Abstract
Immunofluorescent microscopy enables the examination of cellular expression and localization of proteins. Cellular localization can often impact protein function, as certain molecular interactions occur in specific cellular compartments. Here we describe in detail the processes necessary for identifying phosphatases in the cell through immunofluorescent microscopy. Identification of phosphatase expression and localization could lead to the discovery of protein function in disease states along with potential substrates and binding partners.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Pages | 111-122 |
Number of pages | 12 |
DOIs | |
State | Published - 2024 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2743 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2024, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Keywords
- Antibody
- Confocal
- Immunofluorescence
- Localization
- Microscopy
- Protein expression
ASJC Scopus subject areas
- Molecular Biology
- Genetics