Examining Phosphatases Through Immunofluorescent Microscopy

Caroline N. Smith, Jessica S. Blackburn

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Immunofluorescent microscopy enables the examination of cellular expression and localization of proteins. Cellular localization can often impact protein function, as certain molecular interactions occur in specific cellular compartments. Here we describe in detail the processes necessary for identifying phosphatases in the cell through immunofluorescent microscopy. Identification of phosphatase expression and localization could lead to the discovery of protein function in disease states along with potential substrates and binding partners.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages12
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2024, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


  • Antibody
  • Confocal
  • Immunofluorescence
  • Localization
  • Microscopy
  • Protein expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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