Exercise-driven metabolic pathways in healthy cartilage

A. D. Blazek; J. Nam; R. Gupta; M. Pradhan; P. Perera; N. L. Weisleder; T. E. Hewett; A. M. Chaudhari; B. S. Lee; B. Leblebicioglu; T. A. Butterfield; S. Agarwal

doi:10.1016/j.joca.2016.02.004

Exercise-driven metabolic pathways in healthy cartilage

A. D. Blazek, J. Nam, R. Gupta, M. Pradhan, P. Perera, N. L. Weisleder, T. E. Hewett, A. M. Chaudhari, B. S. Lee, B. Leblebicioglu, T. A. Butterfield, S. Agarwal

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Objective: Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. Methods: Transcriptome-wide gene expression in the articular cartilage of healthy Sprague-Dawley female rats exercised daily (low intensity treadmill walking) for 2, 5, or 15 days was compared to that of non-exercised rats, using Affymetrix GeneChip arrays. Database for Annotation, Visualization and Integrated Discovery (DAVID) was used for Gene Ontology (GO)-term enrichment and Functional Annotation analysis of differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genome (KEGG) pathway mapper was used to identify the metabolic pathways regulated by exercise. Results: Microarray analysis revealed that exercise-induced 644 DEGs in healthy articular cartilage. The DAVID bioinformatics tool demonstrated high prevalence of functional annotation clusters with greater enrichment scores and GO-terms associated with extracellular matrix (ECM) biosynthesis/remodeling and inflammation/immune response. The KEGG database revealed that exercise regulates 147 metabolic pathways representing molecular interaction networks for Metabolism, Genetic Information Processing, Environmental Information Processing, Cellular Processes, Organismal Systems, and Diseases. These pathways collectively supported the complex regulation of the beneficial effects of exercise on the cartilage. Conclusions: Overall, the findings highlight that exercise is a robust transcriptional regulator of a wide array of metabolic pathways in healthy cartilage. The major actions of exercise involve ECM biosynthesis/cartilage strengthening and attenuation of inflammatory pathways to provide prophylaxis against onset of arthritic diseases in healthy cartilage.

Original language	English
Pages (from-to)	1210-1222
Number of pages	13
Journal	Osteoarthritis and Cartilage
Volume	24
Issue number	7
DOIs	https://doi.org/10.1016/j.joca.2016.02.004
State	Published - Jul 1 2016

Bibliographical note

Publisher Copyright:
© 2016 Osteoarthritis Research Society International.

Funding

The funding sources were: National Institutes of Health , Bethesda, MD. Grant numbers AR048781 (S.A.), DE015399 (S.A.), AR063084 (N.W.), 32DEO14320 (J.N.) University of California , Riverside Initial Complement Funds (J.N.), Ohio State University Physiology and Cell Biology , Margaret T. Nishikawara Merit Scholarship Fund (A.D.B.). None of the study sponsors took part in the study design, collection, analysis and interpretation of data, writing of the manuscript, or in the decision of submitting the manuscript for publication.

Funders	Funder number
University of California , Riverside Initial Complement Funds
National Institute of Dental and Craniofacial Research	R01DE015399
Ohio Water Resources Center, Ohio State University

Keywords

Cartilage
Exercise
Gene expression
Metabolic pathways
Osteoarthritis

ASJC Scopus subject areas

Rheumatology
Biomedical Engineering
Orthopedics and Sports Medicine

Access to Document

10.1016/j.joca.2016.02.004

Cite this

@article{45785b6e131c4299b0c73b7ec0703342,

title = "Exercise-driven metabolic pathways in healthy cartilage",

abstract = "Objective: Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. Methods: Transcriptome-wide gene expression in the articular cartilage of healthy Sprague-Dawley female rats exercised daily (low intensity treadmill walking) for 2, 5, or 15 days was compared to that of non-exercised rats, using Affymetrix GeneChip arrays. Database for Annotation, Visualization and Integrated Discovery (DAVID) was used for Gene Ontology (GO)-term enrichment and Functional Annotation analysis of differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genome (KEGG) pathway mapper was used to identify the metabolic pathways regulated by exercise. Results: Microarray analysis revealed that exercise-induced 644 DEGs in healthy articular cartilage. The DAVID bioinformatics tool demonstrated high prevalence of functional annotation clusters with greater enrichment scores and GO-terms associated with extracellular matrix (ECM) biosynthesis/remodeling and inflammation/immune response. The KEGG database revealed that exercise regulates 147 metabolic pathways representing molecular interaction networks for Metabolism, Genetic Information Processing, Environmental Information Processing, Cellular Processes, Organismal Systems, and Diseases. These pathways collectively supported the complex regulation of the beneficial effects of exercise on the cartilage. Conclusions: Overall, the findings highlight that exercise is a robust transcriptional regulator of a wide array of metabolic pathways in healthy cartilage. The major actions of exercise involve ECM biosynthesis/cartilage strengthening and attenuation of inflammatory pathways to provide prophylaxis against onset of arthritic diseases in healthy cartilage.",

keywords = "Cartilage, Exercise, Gene expression, Metabolic pathways, Osteoarthritis",

author = "Blazek, {A. D.} and J. Nam and R. Gupta and M. Pradhan and P. Perera and Weisleder, {N. L.} and Hewett, {T. E.} and Chaudhari, {A. M.} and Lee, {B. S.} and B. Leblebicioglu and Butterfield, {T. A.} and S. Agarwal",

note = "Publisher Copyright: {\textcopyright} 2016 Osteoarthritis Research Society International.",

year = "2016",

month = jul,

day = "1",

doi = "10.1016/j.joca.2016.02.004",

language = "English",

volume = "24",

pages = "1210--1222",

number = "7",

}

TY - JOUR

T1 - Exercise-driven metabolic pathways in healthy cartilage

AU - Blazek, A. D.

AU - Nam, J.

AU - Gupta, R.

AU - Pradhan, M.

AU - Perera, P.

AU - Weisleder, N. L.

AU - Hewett, T. E.

AU - Chaudhari, A. M.

AU - Lee, B. S.

AU - Leblebicioglu, B.

AU - Butterfield, T. A.

AU - Agarwal, S.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Objective: Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. Methods: Transcriptome-wide gene expression in the articular cartilage of healthy Sprague-Dawley female rats exercised daily (low intensity treadmill walking) for 2, 5, or 15 days was compared to that of non-exercised rats, using Affymetrix GeneChip arrays. Database for Annotation, Visualization and Integrated Discovery (DAVID) was used for Gene Ontology (GO)-term enrichment and Functional Annotation analysis of differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genome (KEGG) pathway mapper was used to identify the metabolic pathways regulated by exercise. Results: Microarray analysis revealed that exercise-induced 644 DEGs in healthy articular cartilage. The DAVID bioinformatics tool demonstrated high prevalence of functional annotation clusters with greater enrichment scores and GO-terms associated with extracellular matrix (ECM) biosynthesis/remodeling and inflammation/immune response. The KEGG database revealed that exercise regulates 147 metabolic pathways representing molecular interaction networks for Metabolism, Genetic Information Processing, Environmental Information Processing, Cellular Processes, Organismal Systems, and Diseases. These pathways collectively supported the complex regulation of the beneficial effects of exercise on the cartilage. Conclusions: Overall, the findings highlight that exercise is a robust transcriptional regulator of a wide array of metabolic pathways in healthy cartilage. The major actions of exercise involve ECM biosynthesis/cartilage strengthening and attenuation of inflammatory pathways to provide prophylaxis against onset of arthritic diseases in healthy cartilage.

AB - Objective: Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. Methods: Transcriptome-wide gene expression in the articular cartilage of healthy Sprague-Dawley female rats exercised daily (low intensity treadmill walking) for 2, 5, or 15 days was compared to that of non-exercised rats, using Affymetrix GeneChip arrays. Database for Annotation, Visualization and Integrated Discovery (DAVID) was used for Gene Ontology (GO)-term enrichment and Functional Annotation analysis of differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genome (KEGG) pathway mapper was used to identify the metabolic pathways regulated by exercise. Results: Microarray analysis revealed that exercise-induced 644 DEGs in healthy articular cartilage. The DAVID bioinformatics tool demonstrated high prevalence of functional annotation clusters with greater enrichment scores and GO-terms associated with extracellular matrix (ECM) biosynthesis/remodeling and inflammation/immune response. The KEGG database revealed that exercise regulates 147 metabolic pathways representing molecular interaction networks for Metabolism, Genetic Information Processing, Environmental Information Processing, Cellular Processes, Organismal Systems, and Diseases. These pathways collectively supported the complex regulation of the beneficial effects of exercise on the cartilage. Conclusions: Overall, the findings highlight that exercise is a robust transcriptional regulator of a wide array of metabolic pathways in healthy cartilage. The major actions of exercise involve ECM biosynthesis/cartilage strengthening and attenuation of inflammatory pathways to provide prophylaxis against onset of arthritic diseases in healthy cartilage.

KW - Cartilage

KW - Exercise

KW - Gene expression

KW - Metabolic pathways

KW - Osteoarthritis

UR - http://www.scopus.com/inward/record.url?scp=84961226647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961226647&partnerID=8YFLogxK

U2 - 10.1016/j.joca.2016.02.004

DO - 10.1016/j.joca.2016.02.004

M3 - Article

C2 - 26924420

AN - SCOPUS:84961226647

SN - 1063-4584

VL - 24

SP - 1210

EP - 1222

JO - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

IS - 7

ER -

Exercise-driven metabolic pathways in healthy cartilage

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this