Exonuclease 1-dependent and independent mismatch repair

Eva M. Goellner, Christopher D. Putnam, Richard D. Kolodner

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

DNA mismatch repair (MMR) acts to repair mispaired bases resulting from misincorporation errors during DNA replication and also recognizes mispaired bases in recombination (HR) intermediates. Exonuclease 1 (Exo1) is a 5'. →. 3' exonuclease that participates in a number of DNA repair pathways. Exo1 was identified as an exonuclease that participates in Saccharomyces cerevisiae and human MMR where it functions to excise the daughter strand after mispair recognition, and additionally Exo1 functions in end resection during HR. However, Exo1 is not absolutely required for end resection during HR in vivo. Similarly, while Exo1 is required in MMR reactions that have been reconstituted in vitro, genetics studies have shown that it is not absolutely required for MMR in vivo suggesting the existence of Exo1-independent and Exo1-dependent MMR subpathways. Here, we review what is known about the Exo1-independent and Exo1-dependent subpathways, including studies of mutations in MMR genes that specifically disrupt either subpathway.

Original languageEnglish
Pages (from-to)24-32
Number of pages9
JournalDNA Repair
Volume32
DOIs
StatePublished - Aug 1 2015

Bibliographical note

Funding Information:
This work was supported by NIH Grant R01GM50006 to R.D.K., NIH NSRA F32GM106598 to E.M.G., and the Ludwig Institute for Cancer Research to R.D.K. and C.D.P.

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

  • DNA replication fidelity
  • Excision
  • Mispaired base
  • Mlh1-Pms1
  • Msh2-Msh6
  • Mutagenesis
  • Recombination

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Exonuclease 1-dependent and independent mismatch repair'. Together they form a unique fingerprint.

Cite this