TY - JOUR
T1 - Experimental infection with neuropathogenic equid herpesvirus type 1 (EHV-1) in adult horses
AU - Goehring, Lutz S.
AU - Van Maanen, C.
AU - Berendsen, Marcel
AU - Cullinane, Ann
AU - De Groot, Raoul J.
AU - Rottier, Peter J.M.
AU - Wesselingh, Jeroen J.C.M.
AU - Sloet van Oldruitenborgh-Oosterbaan, Marianne M.
PY - 2010/11
Y1 - 2010/11
N2 - Equid herpesvirus type 1 (EHV-1)-associated myeloencephalopathy (EHM) may follow an infection with the virus in horses. This study tested three hypotheses: (1) a large inhaled dose of a neuropathogenic EHV-1 strain would induce a cell-associated viraemia in all infected horses; (2) neurological disease will only occur in viraemic horses, and (3) the cerebrospinal fluid (CSF) composition following EHV-1 viraemia will be an indicator for EHM.Four EHV-1 seronegative horses were inoculated with EHV-1 by inhalation. Three developed clinical signs of neurological disease, which were mild in two horses and lacking typical EHM histopathological findings, but moderately severe in the third horse. This latter animal was the only one found to be viraemic, with xanthochromic CSF and spinal cord histopathology findings characteristic of EHM. This study showed that cell-associated viraemia was not guaranteed, despite a large-dose inoculation with EHV-1, yet viraemia was probably a pre-requisite for subsequent development of EHM. The histopathological changes used to confirm EHM may be predicted from CSF analysis.
AB - Equid herpesvirus type 1 (EHV-1)-associated myeloencephalopathy (EHM) may follow an infection with the virus in horses. This study tested three hypotheses: (1) a large inhaled dose of a neuropathogenic EHV-1 strain would induce a cell-associated viraemia in all infected horses; (2) neurological disease will only occur in viraemic horses, and (3) the cerebrospinal fluid (CSF) composition following EHV-1 viraemia will be an indicator for EHM.Four EHV-1 seronegative horses were inoculated with EHV-1 by inhalation. Three developed clinical signs of neurological disease, which were mild in two horses and lacking typical EHM histopathological findings, but moderately severe in the third horse. This latter animal was the only one found to be viraemic, with xanthochromic CSF and spinal cord histopathology findings characteristic of EHM. This study showed that cell-associated viraemia was not guaranteed, despite a large-dose inoculation with EHV-1, yet viraemia was probably a pre-requisite for subsequent development of EHM. The histopathological changes used to confirm EHM may be predicted from CSF analysis.
KW - Adult horse
KW - Cerebrospinal fluid analysis
KW - Infection experiment
KW - Neuropathogenic EHV-1
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U2 - 10.1016/j.tvjl.2009.08.007
DO - 10.1016/j.tvjl.2009.08.007
M3 - Article
C2 - 19726209
AN - SCOPUS:78149408712
VL - 186
SP - 180
EP - 187
IS - 2
ER -