TY - JOUR
T1 - Exploiting the reversibility of natural product glycosyltransferase- catalyzed reactions
AU - Zhang, Changsheng
AU - Griffith, Byron R.
AU - Fu, Qiang
AU - Albermann, Christoph
AU - Fu, Xun
AU - Lee, In Kyoung
AU - Li, Lingjun
AU - Thorson, Jon S.
PY - 2006/9/1
Y1 - 2006/9/1
N2 - Glycosyltransferases (GTs), an essential class of ubiquitous enzymes, are generally perceived as unidirectional catalysts. In contrast, we report that four glycosyltransferases from two distinct natural product biosynthetic pathways-calicheamicin and vancomycin-readily catalyze reversible reactions, allowing sugars and aglycons to be exchanged with ease. As proof of the broader applicability of these new reactions, more than 70 differentially glycosylated calicheamicin and vancomycin variants are reported. This study suggests the reversibility of GT-catalyzed reactions may be general and useful for generating exotic nucleotide sugars, establishing in vitro GT activity in complex systems, and enhancing natural product diversity.
AB - Glycosyltransferases (GTs), an essential class of ubiquitous enzymes, are generally perceived as unidirectional catalysts. In contrast, we report that four glycosyltransferases from two distinct natural product biosynthetic pathways-calicheamicin and vancomycin-readily catalyze reversible reactions, allowing sugars and aglycons to be exchanged with ease. As proof of the broader applicability of these new reactions, more than 70 differentially glycosylated calicheamicin and vancomycin variants are reported. This study suggests the reversibility of GT-catalyzed reactions may be general and useful for generating exotic nucleotide sugars, establishing in vitro GT activity in complex systems, and enhancing natural product diversity.
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U2 - 10.1126/science.1130028
DO - 10.1126/science.1130028
M3 - Article
C2 - 16946071
AN - SCOPUS:33748302743
SN - 0036-8075
VL - 313
SP - 1291
EP - 1294
JO - Science
JF - Science
IS - 5791
ER -