Exploring the Role of G Protein Expression in Sodium Butyrate-Enhanced Pancreas Development of Dairy Calves: A Proteomic Perspective

Donglin Wu; Zhanhe Zhang; Xing Wang; David L. Harmon; Yang Jia; Jingwei Qi; Xintong Li; Haobin Jia; Ming Xu

doi:10.1021/acs.jafc.3c08405

Exploring the Role of G Protein Expression in Sodium Butyrate-Enhanced Pancreas Development of Dairy Calves: A Proteomic Perspective

Donglin Wu
, Zhanhe Zhang
, Xing Wang
, David L. Harmon
, Yang Jia
, Jingwei Qi
, Xintong Li
, Haobin Jia
, Ming Xu

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The present study evaluated the effects of sodium butyrate (SB) supplementation on exocrine and endocrine pancreatic development in dairy calves. Fourteen male Holstein calves were alimented with either milk or milk supplemented with SB for 70 days. Pancreases were collected for analysis including staining, immunofluorescence, electron microscopy, qRT-PCR, Western blotting, and proteomics. Results indicated increased development in the SB group with increases in organ size, protein levels, and cell growth. There were also exocrine enhancements manifested as higher enzyme activities and gene expressions along with larger zymogen granules. Endocrine benefits included elevated gene expression, more insulin secretion, and larger islets, indicating a rise in β-cell proliferation. Proteomics and pathway analyses pinpointed the G protein subunit alpha-15 as a pivotal factor in pancreatic and insulin secretion pathways. Overall, SB supplementation enhances pancreatic development by promoting its exocrine and endocrine functions through G protein regulation in dairy calves.

Original language	English
Pages (from-to)	5645-5658
Number of pages	14
Journal	Journal of Agricultural and Food Chemistry
Volume	72
Issue number	11
DOIs	https://doi.org/10.1021/acs.jafc.3c08405
State	Published - Mar 20 2024

Bibliographical note

Publisher Copyright:
© 2024 American Chemical Society.

Funding

The authors are grateful for the funds from the Science and Technology Major Project of Inner Mongolia Autonomous Region [grant number 2020ZD0004] and Natural Science Foundation of Inner Mongolia Autonomous Region [grant number 2023LHMS03048] of the Department of Science and Technology of Inner Mongolia Autonomous Region. They thank the staff at their laboratory for their ongoing assistance.

Funders	Funder number
Department of Science and Technology of Inner Mongolia Autonomous Region
Science and Technology Major Project of Inner Mongolia	2020ZD0004
Science and Technology Major Project of Inner Mongolia
Natural Science Foundation of Inner Mongolia Autonomous Region	2023LHMS03048
Natural Science Foundation of Inner Mongolia Autonomous Region

Keywords

GNA15
endocrine
exocrine
insulin secretion
pancreatic secretion
proteome

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/acs.jafc.3c08405

Cite this

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title = "Exploring the Role of G Protein Expression in Sodium Butyrate-Enhanced Pancreas Development of Dairy Calves: A Proteomic Perspective",

abstract = "The present study evaluated the effects of sodium butyrate (SB) supplementation on exocrine and endocrine pancreatic development in dairy calves. Fourteen male Holstein calves were alimented with either milk or milk supplemented with SB for 70 days. Pancreases were collected for analysis including staining, immunofluorescence, electron microscopy, qRT-PCR, Western blotting, and proteomics. Results indicated increased development in the SB group with increases in organ size, protein levels, and cell growth. There were also exocrine enhancements manifested as higher enzyme activities and gene expressions along with larger zymogen granules. Endocrine benefits included elevated gene expression, more insulin secretion, and larger islets, indicating a rise in β-cell proliferation. Proteomics and pathway analyses pinpointed the G protein subunit alpha-15 as a pivotal factor in pancreatic and insulin secretion pathways. Overall, SB supplementation enhances pancreatic development by promoting its exocrine and endocrine functions through G protein regulation in dairy calves.",

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author = "Donglin Wu and Zhanhe Zhang and Xing Wang and Harmon, \{David L.\} and Yang Jia and Jingwei Qi and Xintong Li and Haobin Jia and Ming Xu",

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AU - Wu, Donglin

AU - Zhang, Zhanhe

AU - Wang, Xing

AU - Harmon, David L.

AU - Jia, Yang

AU - Qi, Jingwei

AU - Li, Xintong

AU - Jia, Haobin

AU - Xu, Ming

PY - 2024/3/20

Y1 - 2024/3/20

N2 - The present study evaluated the effects of sodium butyrate (SB) supplementation on exocrine and endocrine pancreatic development in dairy calves. Fourteen male Holstein calves were alimented with either milk or milk supplemented with SB for 70 days. Pancreases were collected for analysis including staining, immunofluorescence, electron microscopy, qRT-PCR, Western blotting, and proteomics. Results indicated increased development in the SB group with increases in organ size, protein levels, and cell growth. There were also exocrine enhancements manifested as higher enzyme activities and gene expressions along with larger zymogen granules. Endocrine benefits included elevated gene expression, more insulin secretion, and larger islets, indicating a rise in β-cell proliferation. Proteomics and pathway analyses pinpointed the G protein subunit alpha-15 as a pivotal factor in pancreatic and insulin secretion pathways. Overall, SB supplementation enhances pancreatic development by promoting its exocrine and endocrine functions through G protein regulation in dairy calves.

AB - The present study evaluated the effects of sodium butyrate (SB) supplementation on exocrine and endocrine pancreatic development in dairy calves. Fourteen male Holstein calves were alimented with either milk or milk supplemented with SB for 70 days. Pancreases were collected for analysis including staining, immunofluorescence, electron microscopy, qRT-PCR, Western blotting, and proteomics. Results indicated increased development in the SB group with increases in organ size, protein levels, and cell growth. There were also exocrine enhancements manifested as higher enzyme activities and gene expressions along with larger zymogen granules. Endocrine benefits included elevated gene expression, more insulin secretion, and larger islets, indicating a rise in β-cell proliferation. Proteomics and pathway analyses pinpointed the G protein subunit alpha-15 as a pivotal factor in pancreatic and insulin secretion pathways. Overall, SB supplementation enhances pancreatic development by promoting its exocrine and endocrine functions through G protein regulation in dairy calves.

KW - GNA15

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KW - exocrine

KW - insulin secretion

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KW - proteome

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Exploring the Role of G Protein Expression in Sodium Butyrate-Enhanced Pancreas Development of Dairy Calves: A Proteomic Perspective

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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