Expressed gene sequence of the IFNγ-response chemokine CXCL9 of cattle, horses, and swine

Edward Hudgens, Dannielle Tompkins, Patricia Boyd, Joan K. Lunney, David Horohov, Cynthia L. Baldwin

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


This report describes the cloning and characterization of expressed gene sequences of bovine, equine, and swine CXCL9 from RNA obtained from peripheral blood mononuclear cells (PBMC) and other tissues. The bovine coding region was 378 nucleotides in length, while the equine and swine coding regions were 381 nucleotides. Mapping showed that all three sequences were coded for in four exons in the genome, as are the human and mouse genes. The bovine, equine, and swine coding regions shared 83%, 86%, and 84% homology with human CXCL9, respectively, and all three were 74% homologous with mouse CXCL9. Cladogram comparison of the nucleotide sequences of CXCL9 showed that the bovine, equine and swine sequences were more closely related to one another than to either the human or the mouse sequences. However, the human sequence was more closely related to them than it was to the mouse sequence. These relationships were preserved when the deduced amino acid sequences were evaluated and all sequences showed conservation of the characteristic four cysteines. This work sets the stage for further work with these molecules; an integral goal of the U.S. Veterinary Immune Reagent Network is to develop reagents for investigating diseases in livestock species, poultry, and fish.

Original languageEnglish
Pages (from-to)317-321
Number of pages5
JournalVeterinary Immunology and Immunopathology
Issue number3-4
StatePublished - Jun 15 2011

Bibliographical note

Funding Information:
We thank Vanessa Mailloux and Carolyn Herzig for technical assistance with the preparation of cells. Funding for this work was provided by USDA NIFA competitive grants program Grant #2006-35204-16880 for the “ US Veterinary Immune Reagent Network ” ( ).


  • CXCL9
  • Chemokines
  • Equine
  • Ruminant
  • Swine
  • T cells
  • Th1

ASJC Scopus subject areas

  • Immunology
  • Veterinary (all)


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