Abstract
This report describes the initial cloning and characterization of the equine interleukin-17 (IL-17) expressed gene sequence from mRNA obtained from equine intestinal tissue and interleukin-23 (IL-23) expressed gene sequence from mRNA obtained from equine peripheral blood mononuclear cells. Equine IL-17 has 462 nucleotides in the translated region, determined by homology with known human and mouse sequences, and shares 84% and 75% identity, respectively. For the deduced amino acid sequences, the identity with human and mouse is 76% and 70%. Equine IL-23 has 579 nucleotides in the translated region. Homology with known human and mouse sequences was determined to be 89% and 77%. Deduced amino acid identities are 89% with the human sequence and 70% with the mouse sequence. The gene sequences were identified as part of the U.S. Veterinary Immune Reagent Network with a goal of developing reagents in order to aid veterinary researchers in the investigation of diseases in livestock species.
Original language | English |
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Pages (from-to) | 309-313 |
Number of pages | 5 |
Journal | Veterinary Immunology and Immunopathology |
Volume | 133 |
Issue number | 2-4 |
DOIs | |
State | Published - Feb 15 2010 |
Bibliographical note
Funding Information:We thank John Loftus for technical assistance with the preparation of cells. Funding for this work was provided by NRI-USDA competitive grants program grant # 2006-35204-16880 for the “US Veterinary Immune Reagent Network” ( www.vetimm.org ).
Keywords
- Cytokines
- Equine
- IL-17
- IL-23
- T cells
- Th17
- Th2
ASJC Scopus subject areas
- Immunology
- General Veterinary