Expression and characterisation of equine interleukin 2 and interleukin 4

Karen Dohmann, Bettina Wagner, David W. Horohov, Wolfgang Leibold

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


In the present study, we describe the expression of equine IL2 and IL4. The cDNA of equine IL2 or IL4 was cloned in a mammalian expression vector, containing c-terminal myc- and six histidines His6-epitopes for recognition and purification of equine cytokines. The vector constructs were used for transfection of chinese hamster ovary (CHO) cells. Purified equine cytokines were characterised by western blotting. Equine IL2 was secreted with a molecular weight of approximately 17.1 kDa, whereas IL4 was expressed in three different sizes of 17.1, 19.6 and 22.1 kDa, probably due to different glycosylation modifications. The biological activities of both cytokines were tested by proliferation assays using leukoagglutinin (LAG) prestimulated equine PBMC. Both, equine IL2 and IL4 induced dose-dependent lymphocyte proliferation. In contrast to IL4, IL2 supported the proliferation of B cells.

Original languageEnglish
Pages (from-to)243-256
Number of pages14
JournalVeterinary Immunology and Immunopathology
Issue number3-4
StatePublished - Dec 29 2000

Bibliographical note

Funding Information:
We thank Dr. D.P. Lunn for the gift of the monoclonal antibodies CVS4 and CVS8. This work was supported by the DFG Graduiertenkolleg: Zell- und Molekularbiologie in der Tiermedizin in Hannover.


  • Biochemical characterisation
  • Horse IL2 and IL4
  • Interleukins
  • Mammalian expression
  • Proliferations assays

ASJC Scopus subject areas

  • Immunology
  • Veterinary (all)


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