The expression of outer surface protein C (OspC) was determined for North American Borrelia burgdorferi isolates HB19, DN127c19-2, 25015 and both low and high culture passage B31. A monoclonal antibody detected the presence of OspC protein in only two isolates, while polyclonal antiserum identified this protein in all five isolates. The ospC gene was cloned and sequenced for isolates HB19, DN127c19-2 and 25015, and compared with the published ospC sequences of other Lyme disease spirochetes. Bothe the nucleotide and amino acid sequences were found to vary as much among isolates from the same geographic area as between isolates of different species.
|Number of pages||6|
|Journal||FEMS Microbiology Letters|
|State||Published - Dec 15 1994|
Bibliographical noteFunding Information:
Our thanks to B. Wilske for providing monoclonal antibody L221F8; to L. Bockenstedt, F. Kantor, K. DePonte and T. Zhu for production of the anti-N40 OspC polyclonal serum; to D. Persing, A. Barbour and M. Bissett for providing bacterial isolates; to P. Rosa for comments on the manuscript: to J. Hoch for the CLUSTAL V program; to D. Beck, F. Coyle, J. Butler and G. Terwilliger for technical assistance. Supported by National Institute of Allergy and Infectious Diseases, NIH, Grants AI26815 and AI30548.
- Borrelia burgdorferi
- Lyme disease
ASJC Scopus subject areas
- Molecular Biology