Expression cloning of a novel farnesylated protein, RDJ2, encoding a DnaJ protein homologue

Douglas A. Andres, Haipeng Shao, Dean C. Crick, Brian S. Finlin

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


The CAAX farnesyltransferase is a heterodimeric enzyme that attaches a farnesyl group to a single cysteine in cellular proteins which terminate in the sequence CAAX, where C is cysteine, A is an aliphatic amino acid, and X is most often methionine or serine. Substrates include the p21(ras) proteins, nuclear lamins, and a series of retinal proteins. To date, a limited number of substrates for the farnesyltransferase have been identified, predominantly by demonstration of the attachment of a farnesyl group to previously identified cDNA clones which encode proteins containing an appropriate carboxyl-terminal tetrapeptide. We describe here the use of a cDNA fusion protein expression library, together with enzymatic in vitro [3H]farnesyl radiolabeling, as a means of identifying novel farnesylated proteins. One candidate cDNA was fully cloned and found to be a homologue of the Escherichia coli heat shock gene dnaJ. The predicted amino acid sequence of this protein was found to terminate with the tetrapeptide Cys-Ala-His-Gln, which conforms to the consensus sequence for recognition by farnesyltransferase, and was shown to undergo in vivo farnesylation. This farnesylated protein, designated RDJ2 (rat DnaJ homologue 2), is a novel and ubiquitously expressed DnaJ homologue and is the newest member of the subfamily of DnaJ-related proteins which are posttranslationally modified by protein farnesylation.

Original languageEnglish
Pages (from-to)113-124
Number of pages12
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Oct 1 1997

Bibliographical note

Funding Information:
1This work was supported in part by NIH Grant EY11231 and aided by Grant IRG-77653 from the American Cancer Society. The GenBank accession number for rat RDJ2 is U95727.


  • DnaJ
  • Farnesyltransferase
  • Protein isoprenylation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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