TY - JOUR
T1 - Expression of a foreign eukaryotic gene in Saccharomyces cerevisiae
T2 - β-galactosidase from Kluyveromyces lactis
AU - Dickson, Robert C.
PY - 1980
Y1 - 1980
N2 - Three recombinant DNA vectors carrying the β-galactosidase structural gene, LAC4, from the yeast Kluyveromyces lactis were constructed and transformed into Saccharomyces cerevisiae. All transformants expressed the β-galactosidase activity of LAC4. However, the level of enzyme activity varied, being highest in cells transformed with vectors which are maintained as multicopy plasmids and lowest in cells transformed with a vector which integrates into chromosomes. Enzyme levels probably reflect gene dosage. LAC4 is very stable when integrated into a chromosome, but unstable when carried on a plasmid. Therefore, stability is a property of the recombinant vector rather than of LAC4, LAC4-coded β-galactosidase synthesized in either S. cerevisiae or in K. lactis is the same as judged by two-dimensional polyacrylamide gel electrophoresis. However, S. cerevisiae transformed with
AB - Three recombinant DNA vectors carrying the β-galactosidase structural gene, LAC4, from the yeast Kluyveromyces lactis were constructed and transformed into Saccharomyces cerevisiae. All transformants expressed the β-galactosidase activity of LAC4. However, the level of enzyme activity varied, being highest in cells transformed with vectors which are maintained as multicopy plasmids and lowest in cells transformed with a vector which integrates into chromosomes. Enzyme levels probably reflect gene dosage. LAC4 is very stable when integrated into a chromosome, but unstable when carried on a plasmid. Therefore, stability is a property of the recombinant vector rather than of LAC4, LAC4-coded β-galactosidase synthesized in either S. cerevisiae or in K. lactis is the same as judged by two-dimensional polyacrylamide gel electrophoresis. However, S. cerevisiae transformed with
KW - 5-bromo-4-chloro-3-indolyl-β-d-galactoside
KW - Ap
KW - LAC4 cannot grow on lactose
KW - Tc
KW - Xgal
KW - ampicillin
KW - probably because lactose does not enter the cell
KW - tetracycline
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U2 - 10.1016/0378-1119(80)90155-9
DO - 10.1016/0378-1119(80)90155-9
M3 - Article
C2 - 6253356
AN - SCOPUS:0019312448
SN - 0378-1119
VL - 10
SP - 347
EP - 356
JO - Gene
JF - Gene
IS - 4
ER -