Abstract
Three recombinant DNA vectors carrying the β-galactosidase structural gene, LAC4, from the yeast Kluyveromyces lactis were constructed and transformed into Saccharomyces cerevisiae. All transformants expressed the β-galactosidase activity of LAC4. However, the level of enzyme activity varied, being highest in cells transformed with vectors which are maintained as multicopy plasmids and lowest in cells transformed with a vector which integrates into chromosomes. Enzyme levels probably reflect gene dosage. LAC4 is very stable when integrated into a chromosome, but unstable when carried on a plasmid. Therefore, stability is a property of the recombinant vector rather than of LAC4, LAC4-coded β-galactosidase synthesized in either S. cerevisiae or in K. lactis is the same as judged by two-dimensional polyacrylamide gel electrophoresis. However, S. cerevisiae transformed with
Original language | English |
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Pages (from-to) | 347-356 |
Number of pages | 10 |
Journal | Gene |
Volume | 10 |
Issue number | 4 |
DOIs | |
State | Published - 1980 |
Keywords
- 5-bromo-4-chloro-3-indolyl-β-d-galactoside
- Ap
- LAC4 cannot grow on lactose
- Tc
- Xgal
- ampicillin
- probably because lactose does not enter the cell
- tetracycline
ASJC Scopus subject areas
- Genetics