Expression of a foreign eukaryotic gene in Saccharomyces cerevisiae: β-galactosidase from Kluyveromyces lactis

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15 Scopus citations

Abstract

Three recombinant DNA vectors carrying the β-galactosidase structural gene, LAC4, from the yeast Kluyveromyces lactis were constructed and transformed into Saccharomyces cerevisiae. All transformants expressed the β-galactosidase activity of LAC4. However, the level of enzyme activity varied, being highest in cells transformed with vectors which are maintained as multicopy plasmids and lowest in cells transformed with a vector which integrates into chromosomes. Enzyme levels probably reflect gene dosage. LAC4 is very stable when integrated into a chromosome, but unstable when carried on a plasmid. Therefore, stability is a property of the recombinant vector rather than of LAC4, LAC4-coded β-galactosidase synthesized in either S. cerevisiae or in K. lactis is the same as judged by two-dimensional polyacrylamide gel electrophoresis. However, S. cerevisiae transformed with

Original languageEnglish
Pages (from-to)347-356
Number of pages10
JournalGene
Volume10
Issue number4
DOIs
StatePublished - 1980

Keywords

  • 5-bromo-4-chloro-3-indolyl-β-d-galactoside
  • Ap
  • LAC4 cannot grow on lactose
  • Tc
  • Xgal
  • ampicillin
  • probably because lactose does not enter the cell
  • tetracycline

ASJC Scopus subject areas

  • Genetics

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