Expression of an intracisternal A-particle-like element in rat ovary

Katherine Michelle Graham, Chemyong Ko, Kyung Soo Park, Kevin Sarge, Ok Kyong Park-Sarge

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


We have isolated a rat intracisternal-A particle element (IAP)-like element (IAP-LE) from ovarian granulosa cells that appears to be identical to the rat EST clone AA964260. The compiled cDNA sequences contain several putative in-frame translation initiation codons with the largest capable of encoding a 365 amino acid protein with a reverse transcriptase domain in the N-terminus as well as a bipartite nuclear localization signal sequence in the middle. Northern blotting shows a major ~7 Kb transcript and a minor ~5 Kb transcript that are abundantly expressed in the ovary. In situ hybridization histochemistry using ovaries from gonadotropin-treated immature rats and regularly cycling adult rats show that this transcript is predominantly localized to granulosa cells of all healthy follicles, including primary follicles, and to newly-formed and healthy corpora lutea. This cell-specific expression pattern of the IAP-LE gene is distinct from those of the several known retroviral elements, suggesting the potentially novel functional importance of the IAP-LE gene. Taken together, our results demonstrate abundant and cell-specific expression of a novel IAP-LE in rat granulosa cells. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)48-57
Number of pages10
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Nov 11 2000

Bibliographical note

Funding Information:
We thank Ms. Lisa Savage for help with in situ hybridization and Dr. Thomas Curry, Jr., for help with follicular morphology. This work was supported by NIH Grants HD30719 and HD36879 to O.K.P.S. O.K.P.S. is a recipient of a NIH Research Career Development Award, HD01135.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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